Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Six36.
Expression pattern : Unlocalized signal was detected

Conversion to Aniseed format of data from Yutaka Satous gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Six36.
Expression pattern : No distinct zygotic signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages, but also somewhat in the later stage.

Conversion to Aniseed format of data from Yutaka Satous gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Six36.
Expression pattern : No distinct zygotic signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages, but also somewhat in the later stage.

Conversion to Aniseed format of data from Yutaka Satous gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Six36.
Expression pattern : No distinct zygotic signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages, but also somewhat in the later stage.

Conversion to Aniseed format of data from Yutaka Satous gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Six36.
Expression pattern : No distinct zygotic signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages, but also somewhat in the later stage.

Aniseed annotation : stronger signal is detected in a-line neural plate.

Vegetal view of a mid gastrula WT embryo probed for Six3/6 expression (red) and electroporated with Dmrt>LacZ (white) .
Original annotation : In Ciona embryos, Six3/6 is specifically expressed in row IV of the 6-row neural plate.

Vegetal view of a mid gastrula WT embryo probed for six3/6 expression (red) and electroporated with Dmrt>LacZ (white). Second picture shows ISH channel alone.
Original annotation : In Ciona embryos, Six3/6 is specifically expressed in row IV of the 6-row neural plate.

In mid gastrula Ciona embryos, Six3/6 is specifically expressed in row IV of the 6-row neural plate.

Vegetal view of a mid gastrula embryo probed for Six3/6 expression (red) and co-electroporated with Dmrt>H2B:YFP (green) and ZicL>LacZ (white).
Original annotation : In Ciona embryos, Six3/6 is specifically expressed in row IV of the 6-row neural plate.

Dorsal and lateral view of a mid gastrula stage wild type embryo probed for Ci-Six3/6 expression. Expression was detected in the anterior neuroectoderm.

Aniseed Comment: update of annotation of (Imai et al., 2004), based on (Imai et al., 2006).

Conversion to Aniseed format of data from Yutaka Satous gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Six36.
Expression pattern : nervous system, unidentifiable.
Comment : Four cells in the neural plate expressed this gene, and two cells posterior to the blastopore also expressed this gene.

Dorsal and lateral views of a late gastrula stage embryo, showing expression of ci-SIX3. Expression of ci-SIX3 was detected by whole-mount in situ hybridization.

At the late gastrula stage, Six3/6 is expressed in row IV of the neural plate.

At the late gastrula stage, Six3/6 is expressed in row IV of neural plate.
Original annotation : The expression of Six3/6 in control embryos at the late gastrula stage (shown in animal view). The expression is illustrated schematically in the lower right by red (palps) and blue (brain).

Dorsal and lateral views of an early neurula stage embryo, showing expression of ci-SIX3. Expression of ci-SIX3 was detected by whole-mount in situ hybridization.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Six36.
Expression pattern : nervous system

Dorsal view of a neurula stage wild type embryo probed for Ci-Six3/6 expression. Expression was detected in the anterior neuroectoderm.

Dorsal view of a late neurula stage wild type embryo probed for Ci-Six3/6 expression. Expression is confined to prospective buccal cavity ectoderm.

Lateral and dorsal views of an early tailbud stage embryo, showing expression of Ci-Six3. Expression of Ci-Six3 was revealed by whole-mount in situ hydridization.

Lateral view of an early tailbud wild type embryo probed for Ci-Six3/6 expression.

Conversion to Aniseed format of data from Yutaka Satous gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Six36.
Expression pattern : nervous system.
Comment : anterior region of the brain

Lateral view of a mid tailbud stage wild type embryo probed for Ci-Six3/6 expression. Expression was confined to prospective buccal cavity ectoderm.

Lateral and dorsal view of an early tailbud wild type embryo probed for Ci-Six3/6 expression. This expression domain encompassed in the presumptive antero-ventral sensory vesicle and the anterior neural boundary (ANB).

Dorsal and lateral views of a mid tailbud stage embryo, showing expression of Ci-Six3/6. Expression of Ci-Six3/6 was detected by whole-mount in situ hybridization.

Conversion to Aniseed format of data from Yutaka Satous gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Six36.
Expression pattern : nervous system.
Comment : anterior region of the brain

Aniseed annotation : no picture available for this stage.

Dorsal view of a late tailbud stage wild type embryo probed for Ci-Six3/6 expression. Expression was confined to prospective buccal cavity ectoderm (arrowhead).

Late tailbud stage wild type embryo probed for Ci-Six3/6 expression. Expression was confined to prospective buccal cavity ectoderm.

Lateral view of a late tailbud wild type embryo probed for Ci-Six3/6 expression. Expression was restricted anteriorly to the sensory vesicle. Open arrowhead: otolith and black arrowhead: ocellus

Late tailbud stage wild type embryo probed for Ci-Six3/6 (red) and Ci-Pitx (blue) expression. The arrow indicates Ci-Six3/6 staining in the pigment cell.

Lateral view of a tadpole stage wild type embryo probed for Ci-Six3/6 expression. Expression was weakly detected anterior and dorsal to the sensory vesicle.