27 results
| Library Name | Percentage | Expression |
|---|---|---|
| Ciona intestinalis whole animal Ciona intestinalis whole animal |
|
0 clone(s) / 0 |
| Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library |
|
7 clone(s) / 5,577 |
| Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult |
|
11 clone(s) / 31,366 |
| Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo |
|
0 clone(s) / 15,579 |
| Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg |
|
0 clone(s) / 31,100 |
| Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva |
|
28 clone(s) / 26,704 |
| Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo |
|
26 clone(s) / 26,680 |
| directional larval cDNA library directional larval cDNA library |
|
0 clone(s) / 39 |
| Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library |
|
0 clone(s) / 0 |
| K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis |
|
0 clone(s) / 5,468 |
| Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library |
|
0 clone(s) / 0 |
| Ciona intestinalis larva Ciona intestinalis larva |
|
0 clone(s) / 0 |
| Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells |
|
0 clone(s) / 29,579 |
| Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle |
|
1 clone(s) / 2,556 |
| Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo |
|
6 clone(s) / 16,939 |
| Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula |
|
6 clone(s) / 25,258 |
| Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad |
|
0 clone(s) / 16,936 |
| Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex |
|
5 clone(s) / 10,463 |
| Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart |
|
70 clone(s) / 13,243 |
| Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland |
|
1 clone(s) / 17,765 |
| Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal |
|
18 clone(s) / 17,872 |
| Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal |
|
127 clone(s) / 107,314 |
| Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal |
|
31 clone(s) / 24,372 |
| Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal |
|
19 clone(s) / 17,126 |
| Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile |
|
0 clone(s) / 3,798 |
| Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx) |
|
1 clone(s) / 2,031 |
| Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) |
|
1 clone(s) / 188,431 |
3 results
OVERVIEW
Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.
Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
|
|
| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 11 (early gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 12 (mid gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 15 (mid neurula) | ||
| C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I) | ||
| C. robusta formely Ciona int. type A, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 11 (early gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 12 (mid gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 15 (mid neurula) | ||
| C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I) | ||
| C. robusta formely Ciona int. type A, Stage 26 (hatching larva) | ||
Ciona robusta adults were obtained from the National Bio-Resource Project for Ciona (NBRP, Japan). 50 unperturbed and Foxd-morphant embryos were collected at the 32-, 64-, and 112-cell stages. RNA was extracted using a Dynabeads mRNA DIRECT Purification Kit (Thermo Fischer Scientific) and libraries were made with an Ion Total RNA-Seq kit ver 2 (Thermo Fischer Scientific). The libraries were sequenced with an Ion PGM instrument (Thermo Fischer Scientific).
| Assay platform | Ion Torrent PGM | |
| Layout | Single-end | |
| Stranded | Yes | |
| Read length | 20-365 | |
| Normalized data types |
|
|
| Wild type | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 6a (early 32-cell) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 10 (112-cell) | ||
| Mutant | ||
| Deregulated molecule(s) | KH2012:KH.C8.890 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 6a (early 32-cell) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 10 (112-cell) | ||
The purpose of this project is to identify genes positively and negatively regulated by signaling molecules that belongs to the TGFbeta superfamily.
| Assay platform | Illumina HiSeq 2500 | |
| Layout | Single-end | |
| Stranded | Yes | |
| Read length | 101 | |
| Normalized data types |
|
|
| Wild type | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
| Mutant 1 | ||
| Deregulated molecule(s) | KH2012:KH.C4.125 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
| Mutant 2 | ||
| Deregulated molecule(s) | KH2012:KH.C2.573 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
53 results
View of a 110-cell stage embryo showing expression of Ci-TnI in muscle precursor cells.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | B8.15 cell pair - B8.7 cell pair - B8.8 cell pair - |
Effects of knockdown of Ci-Tbx6b on the expression of muscle protein genes. Fertilized eggs were injected with Tbx6b and the expression of Ci-TnI was examined at the 110-cell stage.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | B8.15 cell pair - |
Effects of knockdown of Ci-Tbx6c on the expression of muscle protein genes. Fertilized eggs were injected with Tbx6c and the exprcession of Ci-TnI was examined at the 110-cell stage.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | No expression - |
Vegetal view of a 110-cell stage wild type embryo probed for Ci-TroponinI expression. This picture shows expression in the muscle lineage cells.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | B8.15 cell pair - B8.16 cell pair - B8.7 cell pair - B8.8 cell pair - |
Vegetal and lateral views of 110-cell stage embryos probed Ci-TnI expression. Embryos were injected with CiMDFb mRNA into eggs, which were then fertilized and examined for muscle-specific gene (TnI) activity at 110-cell stage. In the injected embryos we can an increase in the level of TnI expression. These pictures show expression of TnI in the muscle (as in control) and in all the B-cell lineage that give rise to endoderm, mesenchyme and notochord.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | whole embryo - |
Vegetal pole view of a 110-cell stage wild type embryo probed for Ci-TnI (troponin I) expression; This picture shows expression in the muscle cells lineage.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | B8.15 cell pair - B8.16 cell pair - B8.7 cell pair - B8.8 cell pair - |
Vegetal pole view of a 110-cell stage embryo probed for Ci-TnI (Troponin I) expression in which Ci-MRF was blocked with Ci-MRF-MO4468. Ci-MRF-MO4468 reduced significantly muscle gene expression of Ci-TnI.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | No expression - |
Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Muscle(secondary lineage) Muscle(primary lineage)
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Muscle(secondary lineage) Muscle(primary lineage)
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Vegetal view of a Ciona robusta embryo whose cell division was arrested during the period from the 64-cell stage to the early tailbud stage, using cytochalasin B. It was electroporated with Bra>LacZ (pTLacZ, control) and probed for Tnl expression.
Original comment: pTLacZ electroporated embryos express TnI only in the primary muscle lineage.
Scale bar: 50μm.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | primary muscle lineage (anterior) - |
Vegetal view of a Ciona robusta embryo whose cell division was arrested during the period from the 64-cell stage to the early tailbud stage, using cytochalasin B. It was electroporated with Bra>MRFa (ppTCiMRFa) and probed for Tnl expression.
TnI is expressed in the primary muscle precursors and ectopically in the notochord precursors.
Original comment: Transcripts encoding TnI were detected in the notochord lineage of embryos electroporated with either pTCiMRFa or pTCiMRFb.
Scale bar: 50μm.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | notochord - primary muscle lineage (anterior) - |
Vegetal view of a Ciona robusta embryo whose cell division was arrested during the period from the 64-cell stage to the early tailbud stage, using cytochalasin B. It was electroporated with Bra>MRFb (ppTCiMRFb) and probed for Tnl expression.
TnI is expressed in the primary muscle precursors and ectopically in the notochord precursors.
Original comment: Transcripts encoding TnI were detected in the notochord lineage of embryos electroporated with either pTCiMRFa or pTCiMRFb.
Scale bar: 50μm.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | notochord - primary muscle lineage - |
Expression of TnI at the early tailbud stage in embryos traited with cytochalasin from the 64 cell stage.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | primary muscle lineage (anterior) - |
Expression of TnI at the early tailbud stage in embryos traited with cytochalasin from the 64 cell stage following injection of CiMRFb mRNA.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | whole embryo - |
Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Muscle(secondary lineage) Muscle(primary lineage)
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Lateral view of a mid-tailbud stage embryo probed with Ci-TnI (Troponin I) expression.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Injection of Ci-Tbx6c mRNA causes an expression of Ci-TnI (Troponin I) in the whole embryo contrary to the control where Ci-TnI is only expressed in tail-muscle cells.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | whole embryo - |
Injection of Ci-Tbx6b mRNA causes an expression of Ci-TnI (Troponin I) in the whole embryo contrary to the control where Ci-TnI is only expressed in tail-muscle cells.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | whole embryo - |
Injection of Ci-Tbx6b MO prevents the expression of Ci-TnI (Troponin I) in a large set of tail muscle cells contrary to the control where Ci-TnI is expressed in all the tail-muscle cells. Only a small pack of cells keeps a normal expression of Ci-TnI.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | primary muscle lineage - |
Injection of Ci-Tbx6c MO lightly reduces the expression of Ci-TnI (Troponin I) in tail-muscle cells.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
View of a mid tailbud stage embryo showing expression of Ci-TnI in muscle cells. Embryos were cleavage arrested in 110-cell stage by injection of cytochalasin B.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Effects of knockdown of Ci-Tbx6b on the expression of muscle protein genes. Fertilized eggs were injected with Ci-Tbx6b MO. Expression was examined at the mid tailbud stage. Embryos were cleavage arrested at the 110-cell stage by injection of cytochalasin B.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Effects of knockdown of Ci-Tbx6c on the expression of muscle protein genes. Fertilized eggs were injected with Ci-Tbx6c MO. Expression was examined at the mid tailbud stage. Embryos were cleavage arrested at the 110-cell stage by injection of cytochalasin B.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Lateral view of a mid tailbud stage embryo, showing expression of Ci-TnI in muscle cells.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Effects of simultaneous knockdown of Ci-Tbx6b/Ci-Tbx6c on the expression of muscle protein genes. The expression of Ci-TnI was examined at the tailbud stage.
Later expression might be activated by mechanisms other tfan these two Tbx6 genes.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | primary muscle lineage - |
Effects of simultaneous knockdown of Ci-Tbx6b/Ci-Tbx6c/Ci-ZicL on the expression of muscle protein genes. The expression of Ci-TnI was examined at the tailbud stage.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | No expression - |
Whole-mount in situ hybridation using digoxigenin-labeled antisense RNA probes. Embryos was reacted with a TnI probe.
Original annotation: as excepted for this muscle specific gene, control embryos accumulated TnI transcripts only in the tail muscle cells, and transcript level appeared to be the same in cells of the two muscle lineages.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Whole-mount in situ hybridization of partial embryos using digoxigenin-labeled antisense RNA probes (B4.1-quarter embryo). Embryo was reacted with a TnI probe.
Original annotation: consistent with the RT/PCR experiments, B4.1-quarter embryos accumulated TnI transcripts at this stage.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | whole embryo - |
Whole-mount in situ hybridization of partial embryos using digoxigenin-labeled antisense RNA probes (three-quarter embryo). Embryo was reacted with a TnI probe.
Original annotation: consistent with the RT/PCR experiments, three-quarter embryos accumulated TnI transcripts at this stage. In three-quarter embryos, these transcripts were localized to a patch of cells near the end of the tail, corresponding to the secondary muscle lineage of normal embryos.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | secondary muscle lineage - |
Whole-mount in situ hybridization of partial embryos using digoxigenin-labeled antisense RNA probes. (three-quarter embryo). Embryo was reacted with a TE45 probe.
Original annotation: the overall appearance of three-quarter embryos in which distinct head and tail regions were seen indicated that these isolates developed normally, as did analysis of TE45 expression, which provided clear evidence of epidermal differenciation.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | whole embryo - |
Mid tailbud embryos were electroporated with a construct containing region -2014bp/66bp upstream Ci-Troponin I (TI construct #1612 in the paper).
No picture available in the article. This construct is unable to drive Ci-Troponin I expression in the muscle probably due to the mutation in the Tbx6, CRE and MyoD sites.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | No expression - |
Mid tailbud embryos were electroporated with a construct containing region -1433bp/66bp upstream Ci-Troponin I (TI construct #1622 in the paper).
No picture available in the article. This construct drives Ci-Troponin I expression in the muscle.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Mid tailbud embryos were electroporated with a construct containing region -775bp/66bp upstream Ci-Troponin I (TI construct #1439 in the paper).
No picture available in the article. This construct drives Ci-Troponin I expression in the muscle.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Mid tailbud embryos were electroporated with a construct containing region -775bp/66bp upstream Ci-Troponin I (TI construct #1519 in the paper).
No picture available in the article. This construct is unable to drive Ci-Troponin I expression in the muscle probably due to the mutation in the MyoD site.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | No expression - |
Mid tailbud embryos were electroporated with a construct containing region -765bp/66bp upstream Ci-Troponin I (TI construct #1491 in the paper).
No picture available in the article. This construct drives Ci-Troponin I expression in the muscle.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Mid tailbud embryos were electroporated with a construct containing region -765bp/66bp upstream Ci-Troponin I (TI construct #1492 in the paper).
No picture available in the article. This construct is unable to drive Ci-Troponin I expression in the muscle probably due to the mutation of Tbx6 and MyoD sites.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | No expression - |
Mid tailbud embryos were electroporated with a construct containing region -757bp/66bp upstream Ci-Troponin I (TI construct #1543 in the paper).
No picture available in the article. This construct drives Ci-Troponin I expression in the muscle.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Mid tailbud embryos were electroporated with a construct containing region -746bp/66bp upstream Ci-Troponin I (TI construct #1518 in the paper).
No picture available in the article. This construct is unable to drive Ci-Troponin I expression in the muscle.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | No expression - |
Mid tailbud embryos were electroporated with a construct containing region -2014bp/66bp upstream Ci-Troponin I (TI construct #1565 in the paper).
Reporter expression was found in the tail muscle.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Mid tailbud embryos were electroporated with a construct containing region -775bp/66bp upstream Ci-Troponin I (TI construct #1493 in the paper).
No picture available in the article. This construct is unable to drive Ci-Troponin I expression in the muscle probably due to the absence of MyoD and CRE regulatory motifs.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | No expression - |
Vegetal view of a Ciona robusta embryo whose cell division was arrested during the period from the 64-cell stage to the early tailbud stage, using cytochalasin B. It was electroporated with Tnl>LacZ (CiTnI500nZ) and control reporter constructs.
LacZ staining is observed in B-line muscle precursors.
Scale bar: 50μm.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | antero central primary muscle lineage (B7.8 line) - |
Vegetal views of Ciona robusta embryos whose cell division was arrested during the period from the 64-cell stage to the early tailbud stage, using cytochalasin B. It was electroporated with Tnl>LacZ (CiTnI500nZ) and Bra>MRFb (pTCiMRFb) overexpression construct.
LacZ staining is observed in B-line muscle precursors and ectopically in notochord precursors.
Original comment: Only pTCiMRFb was able to elicit CiTnI500nZ expression in the notochord (compared to control Bra>LacZ construct).
Scale bar: 50μm.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | primary muscle lineage - primary notochord lineage (A7.3 & A7.7 lines) - |
Vegetal views of Ciona robusta embryos whose cell division was arrested during the period from the 64-cell stage to the early tailbud stage, using cytochalasin B. It was electroporated with Tnl-EboxMut>LacZ (CiTnI500EboxSDMnZ) and Bra>MRFb (pTCiMRFb) overexpression construct.
Original comment: We examined the effect of pTCiMRFb on the activity of CiTnI500EboxSDMnZ and found no
embryos expressing LacZ in the notochord.
Scale bar: 50μm.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | No expression - |
Ciona robusta mid tailbud embryos electroporated with CiTnI(-836/-335bp)>LacZ reporter construct.
LacZ staining is observed in the muscle.
Original comment: CiTnI500nZ-electroporated embryos exhibited robust reporter gene activity in embryonic
muscle.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Ciona robusta mid tailbud embryos electroporated with CiTnI(-836/-335bp)EboxMut>LacZ reporter construct.
No LacZ staining is observed.
Original comment: CiTnI500EboxSDMnZ-electroporated embryos showed significantly lower levels of reporter activity (compared to the unmutated enhancer).
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | No expression - |
Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Muscle(secodary lineage) Muscle(primary lineage)
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | primary muscle lineage - secondary muscle lineage - |
Side view (anterior is to the left) of a late-tailbud embryo stained for Ci-TnI mRNA by in situ hybridization.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - trunk ventral cells (B7.5 line) - |
Lateral pole view of a late tailbud stage wild type embryo probed for Ci-TnI (troponin I) expression. This picture shows expression in the muscle lineage cells.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | primary muscle lineage - secondary muscle lineage - |
Lateral pole view of a late tailbud stage wild type embryo probed for Ci-TnI (Troponin I) expression. This picture shows expression in the tail muscle cell lineage.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Lateral and dorsal pole view of late tailbud stage embryos probed for Ci-TnI (Troponin I) expression in which Ci-MRF expression was blocked by Ci-MRF-MO1640. These picutres show an abnormal tail muscle development whereas the otolith formation was comparable to control.
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Side view (anterior is to the left) of a late-tailbud embryo electroporated with a lacZ transgene containing the -987bp of the 5' flanking Ci-TnI promoter sequence to the -77bp. Staining is restricted to the tail muscles and trunk ventral cells as seen for the endogenous gene (control pannel).
Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates in the paper : -800bp/-100bp
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - trunk ventral cells (B7.5 line) - |
Conversion to Aniseed format of Ghost database entry from Kusakabe T et al., Dev Biol. 2002; 242:188-203. Original annotation: . Expression pattern: Muscle
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) | tail muscles - |
Side view (anterior is to the left) of a young adult (36h after metamorphosis) embryo stained for Ci-TnI mRNA by in situ hybridization. Note staining of the siphon muscles (arrow), along with the heart (arrowhead).
| Stained molecule | KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3) |
| Stained region(s) |
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