27 results
| Library Name | Percentage | Expression |
|---|---|---|
| Ciona intestinalis whole animal Ciona intestinalis whole animal |
|
0 clone(s) / 0 |
| Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library |
|
2 clone(s) / 5,577 |
| Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult |
|
0 clone(s) / 31,366 |
| Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo |
|
9 clone(s) / 15,579 |
| Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg |
|
24 clone(s) / 31,100 |
| Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva |
|
1 clone(s) / 26,704 |
| Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo |
|
21 clone(s) / 26,680 |
| directional larval cDNA library directional larval cDNA library |
|
0 clone(s) / 39 |
| Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library |
|
0 clone(s) / 0 |
| K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis |
|
0 clone(s) / 5,468 |
| Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library |
|
0 clone(s) / 0 |
| Ciona intestinalis larva Ciona intestinalis larva |
|
0 clone(s) / 0 |
| Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells |
|
0 clone(s) / 29,579 |
| Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle |
|
0 clone(s) / 2,556 |
| Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo |
|
9 clone(s) / 16,939 |
| Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula |
|
8 clone(s) / 25,258 |
| Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad |
|
14 clone(s) / 16,936 |
| Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex |
|
0 clone(s) / 10,463 |
| Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart |
|
0 clone(s) / 13,243 |
| Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland |
|
0 clone(s) / 17,765 |
| Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal |
|
7 clone(s) / 17,872 |
| Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal |
|
37 clone(s) / 107,314 |
| Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal |
|
1 clone(s) / 24,372 |
| Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal |
|
10 clone(s) / 17,126 |
| Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile |
|
0 clone(s) / 3,798 |
| Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx) |
|
0 clone(s) / 2,031 |
| Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) |
|
33 clone(s) / 188,431 |
3 results
OVERVIEW
Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.
Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
|
|
| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 11 (early gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 12 (mid gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 15 (mid neurula) | ||
| C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I) | ||
| C. robusta formely Ciona int. type A, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 11 (early gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 12 (mid gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 15 (mid neurula) | ||
| C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I) | ||
| C. robusta formely Ciona int. type A, Stage 26 (hatching larva) | ||
Ciona robusta adults were obtained from the National Bio-Resource Project for Ciona (NBRP, Japan). 50 unperturbed and Foxd-morphant embryos were collected at the 32-, 64-, and 112-cell stages. RNA was extracted using a Dynabeads mRNA DIRECT Purification Kit (Thermo Fischer Scientific) and libraries were made with an Ion Total RNA-Seq kit ver 2 (Thermo Fischer Scientific). The libraries were sequenced with an Ion PGM instrument (Thermo Fischer Scientific).
| Assay platform | Ion Torrent PGM | |
| Layout | Single-end | |
| Stranded | Yes | |
| Read length | 20-365 | |
| Normalized data types |
|
|
| Wild type | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 6a (early 32-cell) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 10 (112-cell) | ||
| Mutant | ||
| Deregulated molecule(s) | KH2012:KH.C8.890 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 6a (early 32-cell) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 10 (112-cell) | ||
The purpose of this project is to identify genes positively and negatively regulated by signaling molecules that belongs to the TGFbeta superfamily.
| Assay platform | Illumina HiSeq 2500 | |
| Layout | Single-end | |
| Stranded | Yes | |
| Read length | 101 | |
| Normalized data types |
|
|
| Wild type | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
| Mutant 1 | ||
| Deregulated molecule(s) | KH2012:KH.C4.125 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
| Mutant 2 | ||
| Deregulated molecule(s) | KH2012:KH.C2.573 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
54 results
Unfertilized egg probed for CiNut expression.The signals are barely detectable suggesting a dispersed distribution of the CiNut maternal transcript.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | whole egg - |
Conversion to Aniseed format of Ghost database entry from Nishikata T et al., Dev Biol. 2001; 238:315-331. Original annotation: weak. Expression pattern: Localized
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | whole embryo - |
Conversion to Aniseed format of Ghost database entry from Nishikata T et al., Dev Biol. 2001; 238:315-331. Original annotation: . Expression pattern: A4.1 a4.2 B4.1 b4.2
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | whole embryo - |
Vegetal view of a gastrula stage wild type embryo probed for CiNut expression.Anterior is up. The signals are barely detectable suggesting a dispersed distribution of the CiNut maternal transcripts.The arrowhead indicates the blastopore.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | whole embryo - |
Neural plate views of mid gastrula stage embryos (30% of development) probed for CiNut expression and stained with SYTO 13.
Original annotation: the CiNut transcript is first detected at this stage in a8.17, a8.19 and a8.25.
Aniseed annotation: according to our anatomical dictionary and to the pictures, its seems that staining is localized in row 2 neural plate (nuclei are ready to cleave in this one), medial 6 cells.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | A9.14 cell pair - A9.16 cell pair - A9.30 cell pair - |
Neural plate views of late gastrula wild type embryos probed for CiNut expression and stained with SYTO 13.
Original annotation: staining is localized in the a-line blastomeres which give rise to the entire brain, and divide into two rows at later stages.
Aniseed annotation: according to our anatomical dictionary and to the pictures, its seems that staining is localized in row 2 neural plate: row 2 has largely cleaved so staining is in both daughters of the medial most 4 cells and the lateral-most cells have not yet cleaved.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | A9.30 cell pair - row II-A neural plate - row II-B neural plate - |
Neural plate views of early neurula wild type embryos probed for CiNut expression and stained with SYTO 13.
Original annotation: A-line nerve cord begin to express CiNut. Several cells in both sides of the posterior neural plate (A9.30 cell pair, A9.32 cell pair and/or their daughter cells which give rise to the posterior neural plate follows.
Aniseed annotation: according to our anatomical dictionary and to the pictures, its seems that staining is localized in all row 2 descendents, judging by the number of cells (bending maybe due to the fact that row 1 has now cleaved). At this stage we can no longer distinguish individual cells well in rows 1-2, as they start to move, whereas rows 3-6 are straight for quite some time (making us think that the staining is row 2).
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | row II neural plate - |
Dorsal views of a mid neurula stage embryo probed for CiNut expression.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | neural plate - |
Neural plate views of neurula wild type embryos probed for CiNut expression and stained with SYTO 13.
Original annotation: all the neural tube cells express CiNut in the neurula. The start of CiNut expression in the nerve cord cells coincides with the posterior cell movement and extensive cell-shape change.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | neural plate - |
Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.
Original annotation:
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | tail nerve cord - visceral ganglion - |
Dorsal view of an initial tailbud stage embryo probed for CiNut expression (b, brain; nc, nerve cord; asterisk expressed presumptive pulp cells).
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.
Original annotation:
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | nervous system - |
Lateral view of an early tailbud probed for CiNut expression (b, brain; nc, nerve cord; asterisk expressed presumptive pulp cells.).
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.
Original annotation:
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | nervous system - |
Mid tailbud embryos probed for reporter expression and electroporated with a construct containing the region upstream of Ci-Nut1 from -123bp to +94bp and inserted into the promoter region of Venus reporter gene. A part of Ci-Nut1 (from -73bp to -3bp) was additionaly ligated to the 5prime of the -123/+94 region (construct named -119/-27 in the paper).
Original Annotation : When NutME + (-73/-3), was used the expression pattern and fluorescence intensity were similar to those for the NutME::Venus construct
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud embryos probed for reporter expression and electroporated with a construct containing the region upstream of Ci-Nut1 from -123bp to +94bp and inserted into the promoter region of Venus reporter gene. A part of Ci-Nut1 (from -73bp to -27bp) was additionaly ligated to the 5prime of the -123/+94 region (construct named -119/-66 in the paper).
Original Annotation : When NutME + (-73/-27), was used the expression pattern and fluorescence intensity were similar to those for the NutME::Venus construct
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - secondary muscle lineage - |
Mid tailbud embryos probed for reporter expression and electroporated with a construct containing the region upstream of Ci-Nut1 from -123bp to +94bp and inserted into the promoter region of Venus reporter gene. A part of Ci-Nut1 (from -51bp to +14bp) was additionaly ligated to the 5prime of the -123/+94 region (construct named -47/-3 in the paper).
Original Annotation : When NutME + (-47/-3) was used, the expression pattern and fluorescence intensity were similar to those for the NutME::Venus construct. When the distal half of the NutME sequence containing the ZicL1 and Fox sites was deleted (-47/-3, in the paper), enhancer activity was almost abolished.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - tail muscles - |
Mid tailbud embryos probed for reporter expression and electroporated with a construct containing the region upstream of Ci-Nut1 from -123bp to +94bp and inserted into the promoter region of Venus reporter gene. A part of Ci-Nut1 (from -123bp to +14bp) was additionaly ligated to the 5prime of the -123/+94 region (construct named -119/-3 in the paper).
Original Annotation : When NutME + (-119/-3) was electroporated into fertilized eggs, embryos demonstrated neural tube-specific expression and ectopic secondary muscle expression.
According to the article, the expression of this construct in the neural tube is enhanced comparing with the 'wild type' region from -123bp to +94bp.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - secondary muscle lineage - |
Mid tailbud embryos probed for reporter expression and electroporated with a construct containing the region upstream of Ci-Nut1 from -123bp to +94bp and inserted into the promoter region of Venus reporter gene. A part of Ci-Nut1 (from -123bp to -10bp) was additionaly ligated to the 5prime of the -123/+94 region (construct named -119/-27 in the paper).
Original Annotation : When NutME + (-119/-27) was electroporated into fertilized eggs, embryos demonstrated neural tube-specific expression and ectopic secondary muscle expression.
According to the article, the expression of this construct in the neural tube is enhanced comparing with the 'wild type' region from -123bp to +94bp.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - secondary muscle lineage - |
Mid tailbud embryos probed for reporter expression and electroporated with a construct containing the region upstream of Ci-Nut1 from -123bp to +94bp and inserted into the promoter region of Venus reporter gene.A part of Ci-Nut1 (from -123bp to -56bp) was additionaly ligated to the 5prime of the -123/+94 region (construct named -119/-66 in the paper).
Original Annotation : When NutME + (-119/-3) was electroporated into fertilized eggs, embryos demonstrated neural tube-specific expression and ectopic secondary muscle expression.
According to the article, the expression of this construct in the neural tube is enhanced comparing with the 'wild type' region from -123bp to +94bp.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - secondary muscle lineage - |
Mid tailbud wild type embryos probed for Ci-Nut expression (no picture available in the paper).
Ci-Nut was expressed in the nervous system.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | nervous system - |
Mid tailbud embryos probed for Ci-Nut expression following the injection of Ci-Rga-MO.
Ci-Nut expression was lost (in 47% of embryos) or reduced (in 41% of embryos) due to the knock-down of Ci-Rga.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | No expression - |
Cross section of tail region of an in situ hybridized mid tailbud embryo.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | tail nerve cord - |
Mid tailbud embryos probed for Venus reporter expression of the Ci-Nut cis regulatory region upstream of Ci-Nut1 from -1030bp to +94bp, construct named -1026bp in the paper.
ANISEED Annotation : Expression was detected in the neural tube.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with a construct containing the region upstream of Ci-Nut1 (named -923bp in the paper) from -927 to +94 and inserted upstream of the Venus reporter gene.
ANISEED Annotation : Reporter expression was detected in the neural tube.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with a construct containing the region upstream of Ci-Nut1 (named -820bp in the paper) from -824 to +94 and inserted upstream of the Venus reporter gene.
ANISEED Annotation : Reporter expression was detected in the neural tube.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with a construct containing the region upstream of Ci-Nut1 (named -729bp in the paper) from -733 to +94 and inserted upstream of the Venus reporter gene.
ANISEED Annotation : Reporter expression was detected in the neural tube.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with a construct containing the region upstream of Ci-Nut1 (named -613bp in the paper) from -617 to +94 and inserted upstream of the Venus reporter gene.
ANISEED Annotation : Reporter expression was detected in the neural tube.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with a construct containing the region upstream of Ci-Nut1 (named -526bp in the paper) from -530 to +94 and inserted upstream of the Venus reporter gene.
ANISEED Annotation : Reporter expression was detected in the neural tube.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with a construct containing the region upstream of Ci-Nut1 (named -422bp in the paper) from -426 to +94 and inserted upstream of the Venus reporter gene.
ANISEED Annotation : Reporter expression was detected in the neural tube.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with a construct containing the region upstream of Ci-Nut1 from -123bp to +94bp, named -119bp or Ci-Nut Minimal Enhancer (Ci-NutME) in the paper, and inserted upstream of the Venus reporter gene.
Original Annotation : Immunological detection of reporter expression. Though ectopic expression in muscle cells derived from A7.8 and faint staining in the notochord and mesenchyme were also observed, the CiNut1 sequence upstream to -123bp was able to drive expression along the entire neural tube.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - notochord - tail muscles - |
Mid tailbud electroporated with a construct containing the region upstream of Ci-Nut1 (named -314bp in the paper) from -318 to +94 and inserted upstream of the Venus reporter gene.
ANISEED Annotation : Reporter expression was detected in the neural tube.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with a construct containing the region upstream of Ci-Nut1 (named -231bp in the paper) from -235 to +94 and inserted upstream of the Venus reporter gene.
ANISEED Annotation : Weak expression was detected in the neural tube.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with the region upstream of Ci-Nut1 from -123bp to +94bp and inserted into the promoter region of Venus reporter gene. In this construct, the Fox binding site was mutated four times.
ANISEED Annotation : Due to mutations, the neural tube expression was reduced but still present.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with the region upstream of Ci-Nut1 from -123bp to +94bp and inserted into the promoter region of Venus reporter gene. In this construct, the first ZicL binding site was mutated (as shown in this picture).
The first picture showed the mutation on the site, and the second picture showing embryos stained.
ANISEED Annotation : Due to the mutation, the neural tube expression was lost in most of the embryos.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | No expression - |
Mid tailbud electroporated with a construct containing the region upstream of Ci-Nut1 (named -73bp in the paper) from -77 to +94 and inserted upstream of the Venus reporter gene.
ANISEED Annotation : This construct can't expression in neural tube but there is a weak expression in the tail muscles.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | tail muscles - |
Mid tailbud electroporated with a construct containing the region upstream of Ci-Nut1 (named -73bp in the paper) from -77 to +94 and inserted upstream of the Venus reporter gene.
ANISEED Annotation : This construct can't expression in neural tube but there is a weak expression in the muscles.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | tail muscles - |
Mid tailbud electroporated with the region upstream of Ci-Nut1 from -123bp to 94bp and inserted into the promoter region of Venus reporter gene. In this construct, the Fox binding site was mutated three times. The first picture showed the mutations on the site, and the second picture showing embryos stained.
ANISEED Annotation : Due to mutations, the neural tube expression was reduced, but still present.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with the region upstream of Ci-Nut1 from -123bp to +94bp and inserted into the promoter region of Venus reporter gene. In this construct, the first ZicL binding site was mutated.
ANISEED Annotation : Diminution of expression in the neural tube.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | No expression - |
Mid tailbud electroporated with the region upstream of Ci-Nut1 from -123bp to +94bp and inserted into the promoter region of Venus reporter gene. In this construct, the Fox binding site was mutated.
ANISEED Annotation : The expression in neural tube is reduced, due to the mutation, but still present.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with the region upstream of Ci-Nut1 from -123bp to +94bp and inserted into the promoter region of Venus reporter gene. In this construct, the Fox binding site was mutated.
ANISEED Annotation : The expression in neural tube is enhanced, due to the mutation.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with the region upstream of Ci-Nut1 from -123bp to 94bp and inserted into the promoter region of Venus reporter gene.
In this construct, the first ZicL binding site was mutated three times.
The first picture showed the mutations on the site, and the second picture showing embryos stained.
ANISEED Annotation : Due to mutations, this contruct can't drive expression in the neural tube.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | No expression - |
Mid tailbud electroporated with the region upstream of Ci-Nut1 from -123bp to +94bp and inserted into the promoter region of Venus reporter gene. In this construct, the first ZicL binding site was mutated.
ANISEED Annotation : The expression in neural tube is reduced, due to the mutation, but still present.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with the region upstream of Ci-Nut1 from -123bp to +94bp and inserted into the promoter region of Venus reporter gene. In this construct, the second ZicL binding site was mutated.
ANISEED Annotation : The expression in neural tube is reduced, due to the mutation, but still present.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with the region upstream of Ci-Nut1 from -123bp to +94bp and inserted into the promoter region of Venus reporter gene. In this construct, the second ZicL binding site was mutated.
ANISEED Annotation : Due to mutations, this contruct can't drive expression in the neural tube in most of the embryos.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | No expression - |
Mid tailbud electroporated with a construct containing the region upstream of Ci-Nut1 (named -47bp in the paper) from -51 to +94 and inserted upstream of the Venus reporter gene.
ANISEED Annotation : This construct can't expression in neural tube but there is a weak expression in the muscles and in the mesenchyme.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | tail muscles - |
Mid tailbud probed for Venus reporter expression of the Ci-Nut cis regulatory region upstream of Ci-Nut1 from -1030bp to +94bp, construct named -1026bp in the paper.
ANISEED Annotation : Expression was detected in the neural tube.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - |
Mid tailbud electroporated with a construct containing the region upstream of Ci-Nut1 (named -119bp, NutME or none in the paper) from -123 to +94 and inserted upstream of the Venus reporter gene.
ANISEED Annotation : Reporter expression was detected in the neural tube, muscle and notochord.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | central nervous system - notochord - tail muscles - |
Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.
Original annotation:
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | whole embryo - |
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) |
Lateral view and cross sections (anterior (o) and posterior (p) cranial region) of a late tailbud stage embryo probed for CiNut expression. (B, brain).
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | anterior sensory vesicle - neck - posterior sensory vesicle - tail nerve cord - visceral ganglion - |
Conversion to aniseed format of DBTGR database entry from Kusakabe team.
Original annotation:
Brain vesicle; visceral ganglion; nerve cord.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | anterior sensory vesicle - posterior sensory vesicle - tail nerve cord - visceral ganglion - |
Tadpole wild type embryo probed for a G protein-coupled receptor-like protein.
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | anterior sensory vesicle - neck - posterior sensory vesicle - visceral ganglion - |
Lateral view of a tadpole embryo probed for CiNut expression (b, brain; vg, visceral ganglion).
| Stained molecule | KH2012:KH.C14.4 (ADRB2; P2RY12; RGR) |
| Stained region(s) | anterior sensory vesicle - neck - posterior sensory vesicle - tail nerve cord - visceral ganglion - |
