Aniseed - Gene Card

EST count

27 results

Library Name	Percentage	Expression
Ciona intestinalis whole animal Ciona intestinalis whole animal		0 clone(s) / 0
Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library		2 clone(s) / 5,577
Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult		0 clone(s) / 31,366
Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo		2 clone(s) / 15,579
Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg		0 clone(s) / 31,100
Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva		0 clone(s) / 26,704
Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo		5 clone(s) / 26,680
directional larval cDNA library directional larval cDNA library		0 clone(s) / 39
Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library		0 clone(s) / 0
K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis		0 clone(s) / 5,468
Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library		0 clone(s) / 0
Ciona intestinalis larva Ciona intestinalis larva		0 clone(s) / 0
Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells		0 clone(s) / 29,579
Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle		0 clone(s) / 2,556
Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo		2 clone(s) / 16,939
Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula		2 clone(s) / 25,258
Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad		0 clone(s) / 16,936
Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex		0 clone(s) / 10,463
Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart		0 clone(s) / 13,243
Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland		0 clone(s) / 17,765
Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal		0 clone(s) / 17,872
Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal		0 clone(s) / 107,314
Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal		3 clone(s) / 24,372
Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal		0 clone(s) / 17,126
Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile		0 clone(s) / 3,798
Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx)		0 clone(s) / 2,031
Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula)		1 clone(s) / 188,431

RNA-Seq transcriptome profiles (by Experiment)

3 results

OVERVIEW

Expression profile summary from all experiments in WT and mutant conditions:

FPKM & RPKM

Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.

Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.

Download FPKM & RPKM data

OVERVIEW

Expression profile summary from all experiments in WT and mutant conditions:

Relative Log Expression RLE

Be careful, RLE and 2^RLE does not permit you to compare a gene across different experiments.

Download RLE Relative Log Expression data

RNA-Seq Experiment n°34135

Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Assay platform	Illumina HiSeq 2000
Layout	Paired-end
Stranded	Yes
Read length	49
Normalized data types	FPKM RLE
Wild type - Replica 1
	*Territories*	whole embryo -

	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
		C. robusta formely Ciona int. type A, Stage 8 (64-cell)
		C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
		C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
		C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
		C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
		C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

Wild type - Replica 2
	*Territories*	whole embryo -

	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
		C. robusta formely Ciona int. type A, Stage 8 (64-cell)
		C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
		C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
		C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
		C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
		C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

RNA-Seq Experiment n°34138

Ciona robusta adults were obtained from the National Bio-Resource Project for Ciona (NBRP, Japan). 50 unperturbed and Foxd-morphant embryos were collected at the 32-, 64-, and 112-cell stages. RNA was extracted using a Dynabeads mRNA DIRECT Purification Kit (Thermo Fischer Scientific) and libraries were made with an Ion Total RNA-Seq kit ver 2 (Thermo Fischer Scientific). The libraries were sequenced with an Ion PGM instrument (Thermo Fischer Scientific).

Assay platform	Ion Torrent PGM
Layout	Single-end
Stranded	Yes
Read length	20-365
Normalized data types	RPKM
Wild type
	*Territories*	whole embryo -

	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)
		C. robusta formely Ciona int. type A, Stage 8 (64-cell)
		C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Mutant
	*Deregulated molecule(s)*	KH2012:KH.C8.890 -
	*Territories*	whole embryo -
	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)
		C. robusta formely Ciona int. type A, Stage 8 (64-cell)
		C. robusta formely Ciona int. type A, Stage 10 (112-cell)

RNA-Seq Experiment n°34139

The purpose of this project is to identify genes positively and negatively regulated by signaling molecules that belongs to the TGFbeta superfamily.

Assay platform	Illumina HiSeq 2500
Layout	Single-end
Stranded	Yes
Read length	101
Normalized data types	RPKM
Wild type
	*Territories*	whole embryo -

	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Mutant 1
	*Deregulated molecule(s)*	KH2012:KH.C4.125 -
	*Territories*	whole embryo -
	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Mutant 2
	*Deregulated molecule(s)*	KH2012:KH.C2.573 -
	*Territories*	whole embryo -
	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

In Situ Experiment Data

79 results

Click here to visualize with MorphoNet

Experiment data ( results)

C. robusta formely Ciona int. type A, Stage 1 (One cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FGF9/16/20.
Expression pattern : No signal was detected

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Vegetal and animal view of a 16-cell stage embryo stained with Fgf9/16/20 expression.
Expression of Fgf9/16/20 is observed in the A5.1, A5.2, B5.1 cell pairs

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Vegetal view of a WT Ciona robusta 16-cell stage embryo probed for Fgf9/16/20 expression
Fgf9/16/20 is expressed in A5.1, A5.2 and B5.1 blastomer pairs.
The brown dashed line separates A-lineage from B-lineage.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Vegetal view of a Ciona robusta 16-cell stage embryo probed for Fgf9/16/20 expression and injected with β-catenin MO.
Fgf9/16/20 expression is lost.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Lateral view, vegetal pole to the right, of a WT Ciona robusta 16-cell stage embryo probed for Fgf9/16/20 expression
Fgf9/16/20 is expressed in A5.1, A5.2 and B5.1.
The green dashed line separates the animal (ectoderm) from the vegetal (mesendoderm) hemispheres and the brown dashed line separates A- (A4.1) and a- (a4.2) lineages from B- (B4.1) and b- (b4.2) lineages.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - A line - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Lateral view, vegetal pole to the rightn of a Ciona robusta 16-cell stage embryo probed for Fgf9/16/20 expression and treated with BIO from the eight-cell stage. BIO is a chemical inhibitor of the upstream inhibitory regulator of β-catenin, GSK-3.
Fgf9/16/20 is expressed in A5.1, A5.2, B5.1, a5.3, a5.4, b5.3 and b5.4 blastomers.
Original comment: Ectopic stabilisation of nβ-catenin resulted in activation of Fgf9/16/20 in ectoderm lineages at the 16-cell stage.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - a5.3 cell pair - a5.4 cell pair - a line - A line - B5.1 cell pair - b5.3 cell pair - b5.4 cell pair - b line -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Animal view of a WT Ciona robusta 16-cell stage embryo probed for Fgf9/16/20 expression.
Fgf9/16/20 is expressed in A5.1 and A5.2 blastomer pairs.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Animal view of a Ciona robusta 16-cell stage embryo probed for Fgf9/16/20 expression and injected with Foxd mRNA.
Fgf9/16/20 is expressed in A5.1 and A5.2 blastomer pairs and the arrowheads indicate weak ectopic expression in a5.3 and a5.4 (ectodermal cells).

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - a5.3 cell pair - a5.4 cell pair - a line - A line -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Animal (left) and vegetal (right) views of a WT Ciona robusta probed for FGF9/16/20 expression at the late 16-cell stage.
FGF9/16/20 is expressed in A5.1, A5.2 and B5.1 blastomere pairs.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Animal (left) and vegetal (right) views of a Ciona robusta Foxd morphant probed for FGF9/16/20 expression at the late 16-cell stage.
FGF9/16/20 is expressed in A5.1, A5.2 and B5.1 blastomere pairs.
Original comment: Fgf9/16/20 is activated independently of Foxd at the 16-cell stage.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Vegetal view of a 16-cell stage Ciona intestinalis embryo stained for FGF9/16/20 mRNA (AB086097) by in situ hybridization. Anterior is up.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FGF9/16/20.
Expression pattern : A5.1, A5.2, B5.1

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Animal and vegetal views of a 16-cell stage embryo probed for FGF9/16/20 expression.
Original annotation: In normal embryos, Fgf9/16/20 is expressed in the VD, and the same expression pattern was observed in embryos injected with a control morpholino oligonucleotide (MO).

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Animal and vegetal views of a 16-cell stage embryo probed for FGF9/16/20 expression, injected with Gata.a-MO.
Original annotation: Fgf9/16/20 expression was scarcely affected in morphants of Gata.a.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Animal and vegetal views of a 16-cell stage embryo probed for FGF9/16/20 expression, injected with beta-catenin-MO.
Original annotation: The expression of Fgf9/16/20 was lost in β-catenin morphants as a previous study has shown (Imai K et al., 2002).

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Animal and vegetal views of a 16-cell stage embryo probed for FGF9/16/20 expression, injected with Zic-r.a MO.
Original annotation: Fgf9/16/20 expression was scarcely affected in morphants of Zic-r.a.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Picture => Image showing Gfp expression, which was revealed by in situ hybridization, in embryos electroporated with the fourth and eighth constructs shown in the first image. “Green boxes indicate the Gfp gene and SV40 polyadenylation signal. The numbers indicate the relative nucleotide positions from the transcription start site of Fgf9/16/20. Mutant Tcf7-binding sites are indicated by X. The graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, posterior vegetal blastomeres, and animal blastomeres. Note that not all cells or embryos could express the reporter because of mosaic incorporation of the electroporated plasmid.”

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

"Green boxes indicate the Gfp gene and SV40 polyadenylation signal. The numbers indicate the relative nucleotide positions from the transcription start site of Fgf9/16/20. The graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, posterior vegetal blastomeres, and animal blastomeres. Note that not all cells or embryos could express the reporter because of mosaic incorporation of the electroporated plasmid."

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Green boxes indicate the Gfp gene and SV40 polyadenylation signal. The numbers indicate the relative nucleotide positions from the transcription start site of Fgf9/16/20. The graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, posterior vegetal blastomeres, and animal blastomeres. Note that not all cells or embryos could express the reporter because of mosaic incorporation of the electroporated plasmid.
Original annotation: Expression in AVD and PVD in most of the embryos.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

“Green boxes indicate the Gfp gene and SV40 polyadenylation signal. The numbers indicate the relative nucleotide positions from the transcription start site of Fgf9/16/20. Mutant Tcf7-binding sites are indicated by X. The graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, posterior vegetal blastomeres, and animal blastomeres. Note that not all cells or embryos could express the reporter because of mosaic incorporation of the electroporated plasmid.”

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Scale bar, 100 μm.
"Replacing the Tcf7 sites critical for expression of Fgf9/16/20 in the anterior and posterior vegetal cells with the proximal Tcf7 site critical for expression of Tbx6.b in the posterior vegetal cells did not affect specificity of the reporter gene expression. Thus, the Tcf7-binding site in the upstream region of Tbx6.b was not likely to be qualitatively different from that of Fgf9/16/20."

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Illustrations on the left depict the constructs. A potential repressive element in the upstream sequence of Tbx6.b was inserted into the upstream sequences of Fgf9/16/20. Graphs on the right show the percentage of blastomeres expressing the reporter gene in the anterior vegetal blastomeres and in the posterior vegetal blastomeres. A graph showing the percentage in the animal hemisphere is not shown, because no embryos expressed the reporter in the animal hemisphere.
"With this insertion, the construct rarely drove reporter expression in the AVD"
"Although the result does not necessarily indicate that the 22 bp region is the only repressive element in the upstream region of Tbx6.b, it showed that this 22 bp region has a repressive activity."

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

First picture
Constructs depicted in the illustrations on the left were injected with or without an MO against Zic-r.a. Graphs on the right show the percentage of blastomeres expressing the reporter gene in the anterior vegetal blastomeres and in the posterior vegetal blastomeres.
Second picture
Photographs of embryo injected with the Fgf9/16/20 reporter construct. Scale bar, 100 μm.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)

Vegetal view of an early 32-cell stage Ciona intestinalis embryo stained for FGF9/16/20 mRNA (AB086097) by in situ hybridization. Anterior is up.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A6.1 cell pair - A6.2 cell pair - A6.3 cell pair - A6.4 cell pair - B6.1 cell pair - B6.2 cell pair -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A6.1 cell pair - A6.2 cell pair - A6.3 cell pair - A6.4 cell pair - A line - B6.1 cell pair -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A6.1 cell pair - A6.2 cell pair - A6.3 cell pair - A6.4 cell pair - A line - B6.1 cell pair -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A6.1 cell pair - A6.2 cell pair - A6.3 cell pair - A6.4 cell pair - A line - B6.1 cell pair -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A6.1 cell pair - A6.2 cell pair - A6.3 cell pair - A6.4 cell pair - A line - B6.1 cell pair -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Vegetal view of a late 32-cell stage wild type embryo, showing expression of Ci-Fgf9/16/20.

Ci-Fgf9/16/20 was expressed in the vegetal hemisphere after 16-cell stage.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A line - B line -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Vegetal view of a late 32-cell stage embryos, showing expression of Ci-Fgf9/16/20 following the injection of Ci-Rga-MO.

Ci-Fgf9/16/20 was still expressed in the vegetal hemisphere for 81% of embryos.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A line - B line -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FGF9/16/20.
Expression pattern : A6.1, A6.2, A6.3, A6.4, B6.1

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A6.1 cell pair - A6.2 cell pair - A6.3 cell pair - A6.4 cell pair - B6.1 cell pair -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Vegetal view of a late 32-cell stage embryo, showing expression of Ci-Fgf9/16/20.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A line - B line -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Vegetal view of a late 32-cell stage embryo showing expression of Ci-Fgf9/16/20, following injection of Ci-ZF278 MO.

Original annotation:
Expression of Ci-FGF9/16/20 became undetectable in embryos injected with morpholino against Ci-ZF278.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Vegetal view of a late 32-cell stage embryo showing expression of Ci-Fgf9/16/20, following injection of Ci-PGAP1 MO.

Original annotation:
Expression of Ci-FGF9/16/20 became undetectable in embryos injected with morpholino against Ci-PGAP1.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Vegetal view of a late 32-cell stage embryo showing expression of Ci-Fgf9/16/20, following injection of Ci-Spatial/C4orf17 MO.

Original annotation:
Expression of Ci-FGF9/16/20 became undetectable in embryos injected with morpholino against Ci-Spatial/C4orf17.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Vegetal view of a late 32-cell stage embryo showing expression of Ci-Fgf9/16/20, following injection of Ci-FLJ10634 MO.

Original annotation:
Expression of Ci-FGF9/16/20 became undetectable in embryos injected with morpholino against Ci-FLJ10634.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Vegetal view of a late 32-cell stage embryo showing expression of Ci-Fgf9/16/20, following injection of Ci-beta-catenin MO.

Original annotation:
Expression of Ci-FGF9/16/20 became undetectable in embryos injected with morpholino against Ci-beta-catenin.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Vegetal view of a late 32-cell stage embryo showing expression of Ci-Fgf9/16/20, following Ci-C10orf11 MO.

Original annotation:
Expression of Ci-FGF9/16/20 became undetectable in embryos injected with morpholino against Ci-C10orf11.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Vegetal view of a WT Ciona robusta late 32-cell stage embryo probed for FGF9/16/20 expression.
Expression is observed in A-lineage and in B6.1 blastomere pair.
In schematic representation of gene expression, notochord and nerve cord precursors mother cells are in purple. Blastomeres with gene expression are marked by dots.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A6.1 cell pair - A6.2 cell pair - A6.3 cell pair - A6.4 cell pair - A line - B6.1 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FGF9/16/20.
Expression pattern : A7.4, A7.8, B7.4

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A7.4 cell pair - A7.8 cell pair - B7.4 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

Vegetal view of a WT Ciona robusta 64-cell stage embryo probed for FGF9/16/20 expression.
Expression is observed in A7.4, A7.8, B7.4 and B7.8 blastomere pairs.
In schematic representation of gene expression, notochord precursors are shown in red and nerve cord precursors in blue. Blastomeres with gene expression are marked by dots.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A7.4 cell pair - A7.8 cell pair - B7.4 cell pair - B7.8 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Original annotation :
Gene name :FGF9/16/20.
Expression pattern : A8.7, A8.8, A8.15, A8.16, B8.7, B8.8

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A8.15 cell pair - A8.16 cell pair - A8.7 cell pair - A8.8 cell pair - B8.7 cell pair - B8.8 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Vegetal view of a 110-cell stage embryo probed for FGF9/16/20 expression following egg micro-injection of a FoxD-a/b MO. FGF9/16/20 was down-regulated in A-line nerve cord cells.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	B8.7 cell pair - B8.8 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Vegetal view of a WT Ciona robusta 112-cell stage embryo probed for FGF9/16/20 expression.
Expression is observed inA8.7, A8.8, A8.15, A8.16, B8.8 and B8.7 blastomere pairs.
In schematic representation of gene expression, notochord precursors are shown in red and nerve cord precursors in blue. Blastomeres with gene expression are marked by dots.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A8.15 cell pair - A8.16 cell pair - A8.7 cell pair - A8.8 cell pair - B8.7 cell pair - B8.8 cell pair -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Late gastrula stage wild type embryo probed for FGF9/16/20 expression. No picture available in the article.

Original Annotation :
FGF9/16/20 was expressed in A9.14 and A9.16 cell pairs.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A9.14 cell pair - A9.16 cell pair -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Neural plate view of a Mid gastrula stage wild type embryo probed for Ci-FGF9/16/20 expression.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A9.14 cell pair - A9.16 cell pair - primary muscle lineage -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Neural plate view of a Mid gastrula stage embryo probed for Ci-FGF9/16/20 expression in which Nodal signalling has been blocked. The marker continued to be expressed in the correct row of neural plate cells, but the expression was expanded laterally to a maximum of eight cells in total. Control sibling embryo shows the wild type pattern of the gene.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	primary muscle lineage - row II neural plate -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Neural plate view of a Mid gastrula stage embryo probed for Ci-FGF9/16/20 expression in which Nodal signalling has been blocked by injection of Nodal MO in eggs. The marker continued to be expressed in the correct row of neural plate cells, but the expression was expanded laterally to a maximum of eight cells in total. Control sibling embryo shows the wild type pattern of the gene.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	primary muscle lineage - row II neural plate -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Vegetal view of a mid gastrula stage embryo probed for Ci-FGF9/16/20.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A9.14 cell pair - A9.16 cell pair - tail muscles -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Vegetal view of a mid gastrula stage embryo probed for Ci-FGF9/16/20 following the treatment of U0126.

Original Annotation : there is ectopic expression of Ci-FGF9/16/20 following the treatment with U0126 (detected in row I).

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A9.13 cell pair - A9.14 cell pair - A9.15 cell pair - A9.16 cell pair - tail muscles -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Vegetative view of an early gastrula. FGF9/16/20 is expressed in A9.14 and A9.16 blastomere pair (medial row II neural plate cells), and in primary muscle lineage.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A9.14 cell pair - A9.16 cell pair - primary muscle lineage -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Vegetative view of a mid gastrula probed for Fgf9/16/20 expression, where Snail is overexpressed in medial lateral neural plate (electroporation of pETR>Snail). Fgf9/16/20 expression is lost in the neural plate.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	primary muscle lineage -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Vegetative view of a mid gastrula probed for Fgf9/16/20 expression and treated with SB431542 (Nodal inhibitor) until early gastrula stage.
Original annotation : SB431542 treatment results in ectopic expression of Fgf9/16/20 in the lateral neural plate (red arrowheads).
Aniseed annotation : It seems that the observed stage is a little bit earlier than the control embryo.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A9.14 cell pair - A9.16 cell pair - A9.30 cell pair - A9.32 cell pair - primary muscle lineage -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Vegetative view of a mid gastrula probed for Fgf9/16/20 expression and treated with SB431542 (Nodal inhibitor) until early gastrula stage, where Snail is overexpressed in medial lateral neural plate (electroporation of pETR>Snail).
Fgf9/16/20 expression is lost in the neural plate.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	primary muscle lineage -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Vegetative view of a mid gastrula probed for Fgf9/16/20 expression, where Nodal is overexpressed in animal territories (electroporation of pFOG>Nodal).
Fgf9/16/20 expression is lost in the neural plate.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	primary muscle lineage -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Neural plate view of a Late gastrula stage wild type embryo probed for Ci-FGF9/16/20 expression.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	a9.34 cell pair - a9.38 cell pair - primary muscle lineage - row II-A neural plate - row II-B neural plate -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FGF9/16/20.
Expression pattern : nervous system

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	neural plate -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

A vegetal view of a late gastrula wild type stage embryo probed for Ci-FGF9/16/20 expression.

Original Annotation : The expression of Ci-FGF9/16/20 was detected in tail muscle, in A9.14 and A9.16.
The numbers indicate the mean number of cells (c) per embryo expressing a given gene, n=total number of embryos analysed.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A10.28 cell pair - A10.32 cell pair - tail muscles -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

A vegetal view of a late gastrula stage embryo probed for Ci-FGF9/16/20 expression following the injection of Delta2-MO.

Original Annotation : The expression of Ci-FGF9/16/20 was not affected by the inhibition of Delta2/Notch signaling.
The numbers indicate the mean number of cells (c) per embryo expressing a given gene, n=total number of embryos analysed.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A10.28 cell pair - A10.32 cell pair - tail muscles -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

A vegetal view of a late gastrula stage embryo probed for Ci-FGF9/16/20 expression following the injection of DAPT.

Original Annotation : The expression of Ci-FGF9/16/20 in neural plate was not affected by the inhibition of Delta2/Notch signaling due to DAPT.
The numbers indicate the mean number of cells (c) per embryo expressing a given gene, n=total number of embryos analysed.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	A10.28 cell pair - A10.32 cell pair - tail muscles -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FGF9/16/20.
Expression pattern : nervous system

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	neural plate -

C. robusta formely Ciona int. type A, Stage 17 (initial tailbud I)

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.

Original annotation:
Expression pattern: Epidermis Endoderm Endodermal strand Nerve cord Brain Papilla Trunk lateral cells.

Aniseed annotation : a staining has been detected in the nervous system but also in the tail nerve cord, the palps and the visceral ganglion. A stronger staining has been detected in the notochord and the mesenchyme.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	endodermal strand - epidermis - head endoderm - nervous system - notochord - trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.

Original annotation:
Expression pattern: Epidermis Nerve cord Brain Papilla Trunk lateral cells.

Aniseed annotation : a staining has been detected in the nervous system but also in the visceral ganglion, the tail nerve cord and the palps.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	epidermis - nervous system - trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Dorsal view of an early tailbud stage wild type embryo probed for FGF9/16/20 expression.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.

Original Annotation :
FGF9/16/20 was expressed in A11.64, A11.63 cell pairs (posterior sensory vesicle) and in A11.62 and A11.61 cell pairs (neck).

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	neck - posterior sensory vesicle -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Lateral view with anterior to the left of an early tailbud stage embryo probed for fgf9/16/20 expression.
Expression was observed in the posterior muscle cells close to the tail tip. (Image from C. Hudson and H. Yasuo)

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	tail muscles -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Lateral view of an early tailbud stage embryo probed for Fgf9/16/20 and Pax2/5/8 expression.

Original annotation
Ci-Fgf9/16/20 is expressed anterior to Ci-Fgf8/17/18, spanning the domain marked by Ci-Pax2/5/8 and the first Ci-En stripe.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	visceral ganglion -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Lateral view of an early tailbud stage embryo probed for En and Fgf9/16/20 expression.

Original annotation :
Three others Fgfs were also found to be expressed in the CNS.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	neck - nervous system -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Lateral view of an early tailbud stage embryo prober for FgfL and Fgf9/16/20 expression.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	neck - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FGF9/16/20.
Expression pattern : nervous system

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	nervous system -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.

Original annotation:
Expression pattern: Epidermis Nerve cord Brain Papilla Trunk lateral cells.

Aniseed annotation : a staining has been detected in the nervous system but also in the tail nerve cord, the palps and the visceral ganglion. A staining has also been detected in the part of the tail epidermis.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	epidermis - nervous system - trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FGF9/16/20.
Expression pattern : nervous system

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	nervous system -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.

Original annotation:
Expression pattern: Epidermis Nerve cord Brain Trunk lateral cells.

Aniseed annotation : a staining has been detected in the nervous system but also in the tail nerve cord and the visceral ganglion. A stronger staining has been detected in the head endoderm.

Stained molecule	KH2012:KH.C2.125 (FGF16; FGF20; FGF9)
Stained region(s)	epidermis - head endoderm - nervous system - trunk lateral cells (A7.6 line) -