Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :ADMP.
Expression pattern : No signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :ADMP.
Expression pattern : B5.1

Illustrations on the left depict the constructs. The numbers indicate the relative nucleotide positions from the transcription start site of Admp. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, in the posterior vegetal blastomeres, and in the animal blastomeres.

Illustrations on the left depict the constructs. The numbers indicate the relative nucleotide positions from the transcription start site of Admp. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, in the posterior vegetal blastomeres, and in the animal blastomeres.

Illustrations on the left depict the constructs. The numbers indicate the relative nucleotide positions from the transcription start site of Admp. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, in the posterior vegetal blastomeres, and in the animal blastomeres.

Illustrations on the left depict the constructs. The numbers indicate the relative nucleotide positions from the transcription start site of Admp. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, in the posterior vegetal blastomeres, and in the animal blastomeres.
"The 323 bp upstream region of Admp was sufficient to drive reporter expression specifically in the PVD at the 16-cell stage"

Illustrations on the left depict the constructs. The numbers indicate the relative nucleotide positions from the transcription start site of Admp. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, in the posterior vegetal blastomeres, and in the animal blastomeres.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :ADMP.
Expression pattern : B6.1, B6.4

Animal and vegetal views of a late 32-cell stage embryo. The hybridization signal for Ci-ADMP was detected in B6.1, B6.2 and B6.4 cell pairs.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :ADMP.
Expression pattern : A7.1, A7.2, A7.5, A7.6, B7.1, B7.2, B7.5, B7.7, B7.8

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :ADMP.
Expression pattern : B7.5, B8.15, B8.16, a8.21, a8.22, a8.23, a8.24, a8.27, a8.28, a8.29, a8.30, a8.31, a8.32

Lateral view of a WT Ciona robusta gastrula embryo probed for Admp (KH.C2.421) expression.
Original annotation : Admp is transiently expressed in all the epidermal cells at the early gastrula stage.
Scale bar : 100 μm.

Sagital view of a mid gastrula stage wild type embryo probed for the BMP ligand ADMP expression.

Original annotation: the BMP ligand ADMP is expressed in the B-line midline vegetal cells, underlying the ventral midline precursors at the mid gastrula stage.

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :ADMP.
Expression pattern : epidermis

Views of late gastrula stage embryos showing reporter expression of the region upstream of ADMP in endoderm. A weak signal was detected in a part of epiderims.

Lateral view of a WT Ciona robusta neurula embryo probed for Admp (KH.C2.421) expression.
Expression is observed in lateral and ventral epidermis.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :ADMP.
Expression pattern : epidermis

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :ADMP.
Expression pattern : epidermis

Lateral view of an early tailbud stage embryo. Expression of ci-ADMP is revealed by whole-mount in situ hybridization. No expression is detected at this stage.

Views of early tailbud stage embryos showing reporter expression of the region upstream of ADMP in palp, brain, a-epidermis, muscle and mesenchyme.

Ventral view of a WT Ciona robusta early tailbud embryo probed for Admp (KH.C2.421) expression.
Expression is observed in lateral epidermis and dorsal endoderm
Insert is a ventral view of the trunk region.

Admp is expressed in lateral epidermis and dorsal endoderm of WT Ciona robusta early tailbud embryos.

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :ADMP.
Expression pattern : epidermis

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Lateral view of a late tailbud stage embryo, showing expression of ci-ADMP in epidermis. Expression of ci-ADMP is revealed by whole-mount in situ hybridization.

Conversion to Aniseed format of Ghost database entry from Kusakabe T et al., Dev Biol. 2002; 242:188-203. Original annotation: . Expression pattern: not clear

Views of larvae showing reporter expression of the region upstream of ADMP in palp, brain, a-epidermis, muscle and mesenchyme.