Aniseed - Gene Card

Gene 'Moocci.CG.ELv1_2.S59...'

EST count

27 results

Library Name	Percentage	Expression
Ciona intestinalis whole animal Ciona intestinalis whole animal		0 clone(s) / 0
Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library		3 clone(s) / 5,577
Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult		1 clone(s) / 31,366
Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo		0 clone(s) / 15,579
Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg		8 clone(s) / 31,100
Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva		0 clone(s) / 26,704
Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo		3 clone(s) / 26,680
directional larval cDNA library directional larval cDNA library		0 clone(s) / 39
Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library		0 clone(s) / 0
K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis		0 clone(s) / 5,468
Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library		0 clone(s) / 0
Ciona intestinalis larva Ciona intestinalis larva		0 clone(s) / 0
Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells		0 clone(s) / 29,579
Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle		0 clone(s) / 2,556
Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo		3 clone(s) / 16,939
Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula		4 clone(s) / 25,258
Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad		1 clone(s) / 16,936
Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex		0 clone(s) / 10,463
Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart		1 clone(s) / 13,243
Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland		1 clone(s) / 17,765
Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal		5 clone(s) / 17,872
Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal		12 clone(s) / 107,314
Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal		0 clone(s) / 24,372
Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal		0 clone(s) / 17,126
Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile		1 clone(s) / 3,798
Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx)		0 clone(s) / 2,031
Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula)		8 clone(s) / 188,431

RNA-Seq transcriptome profiles (by Experiment)

3 results

OVERVIEW

Expression profile summary from all experiments in WT and mutant conditions:

FPKM & RPKM

Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.

Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.

Download FPKM & RPKM data

OVERVIEW

Expression profile summary from all experiments in WT and mutant conditions:

Relative Log Expression RLE

Be careful, RLE and 2^RLE does not permit you to compare a gene across different experiments.

Download RLE Relative Log Expression data

RNA-Seq Experiment n°34135

Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Assay platform	Illumina HiSeq 2000
Layout	Paired-end
Stranded	Yes
Read length	49
Normalized data types	FPKM RLE
Wild type - Replica 1
	*Territories*	whole embryo -

	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
		C. robusta formely Ciona int. type A, Stage 8 (64-cell)
		C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
		C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
		C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
		C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
		C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

Wild type - Replica 2
	*Territories*	whole embryo -

	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
		C. robusta formely Ciona int. type A, Stage 8 (64-cell)
		C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
		C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
		C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
		C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
		C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

RNA-Seq Experiment n°34138

Ciona robusta adults were obtained from the National Bio-Resource Project for Ciona (NBRP, Japan). 50 unperturbed and Foxd-morphant embryos were collected at the 32-, 64-, and 112-cell stages. RNA was extracted using a Dynabeads mRNA DIRECT Purification Kit (Thermo Fischer Scientific) and libraries were made with an Ion Total RNA-Seq kit ver 2 (Thermo Fischer Scientific). The libraries were sequenced with an Ion PGM instrument (Thermo Fischer Scientific).

Assay platform	Ion Torrent PGM
Layout	Single-end
Stranded	Yes
Read length	20-365
Normalized data types	RPKM
Wild type
	*Territories*	whole embryo -

	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)
		C. robusta formely Ciona int. type A, Stage 8 (64-cell)
		C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Mutant
	*Deregulated molecule(s)*	KH2012:KH.C8.890 -
	*Territories*	whole embryo -
	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)
		C. robusta formely Ciona int. type A, Stage 8 (64-cell)
		C. robusta formely Ciona int. type A, Stage 10 (112-cell)

RNA-Seq Experiment n°34139

The purpose of this project is to identify genes positively and negatively regulated by signaling molecules that belongs to the TGFbeta superfamily.

Assay platform	Illumina HiSeq 2500
Layout	Single-end
Stranded	Yes
Read length	101
Normalized data types	RPKM
Wild type
	*Territories*	whole embryo -

	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Mutant 1
	*Deregulated molecule(s)*	KH2012:KH.C4.125 -
	*Territories*	whole embryo -
	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Mutant 2
	*Deregulated molecule(s)*	KH2012:KH.C2.573 -
	*Territories*	whole embryo -
	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

In Situ Experiment Data

48 results

Click here to visualize with MorphoNet

Experiment data ( results)

C. robusta formely Ciona int. type A, Stage 1 (One cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :CAGF9.
Expression pattern : Unlocalized signal was detected.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	whole embryo -

C. robusta formely Ciona int. type A, Stage 4 (8-cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :CAGF9.
Expression pattern : No distinct zygotic signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	whole embryo -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :CAGF9.
Expression pattern : No distinct zygotic signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :CAGF9.
Expression pattern : No distinct zygotic signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :CAGF9.
Expression pattern : No distinct zygotic signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :CAGF9.
Expression pattern : No distinct zygotic signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :CAGF9.
Expression pattern : No distinct zygotic signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :CAGF9.
Expression pattern : No distinct zygotic signal was detected.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Tail tip.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :CAGF9.
Expression pattern : epidermis.
Comment : Epidermal cells expressing this gene are aligned in several lines.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	epidermis -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Ventral and lateral views of an embryo at neurula stage, probed for Tox expression. Original annotation: Expression of Tox in the ventral tail ectoderm was not detectable at the late-gastrula or neurula stage, and began at the tailbud stage in normal embryos.
Aniseed annotation: expression at the tip of the tail.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 16 (late neurula)

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 17 (initial tailbud I)

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: 4hr.. Expression pattern: not clear

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	whole embryo -

C. robusta formely Ciona int. type A, Stage 17 (initial tailbud I)

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline posterior tail epidermis - posterior dorsal midline head epidermis (b8.20 line) - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 17 (initial tailbud I)

Ventral and lateral views of an embryo at initial tailbud stage, probed for Tox expression. Original annotation: expression in ventral tail ectoderm.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline posterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 17 (initial tailbud I)

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline head epidermis - dorsal midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: 4hr.. Expression pattern: not clear

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	whole embryo -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Ventral and dorsal views of a tailbud stage wild type embryo probed for CAGF9 expression.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	central nervous system - dorsal midline anterior tail epidermis - dorsal midline posterior tail epidermis - posterior dorsal midline head epidermis (b8.20 line) - ventral lateral anterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Tailbud stage embryo probed for CAGF9 expression following egg micro-injection of a ADMP MO. CAGF9 is down regulated in ventral part of tail epidermal cells. Expression is not affected in dorsal midline epidermis.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Dorsal view of a tailbud stage embryo probed for CAGF9 expression following egg micro-injection of a Msxb MO. CAGF9 is down-regulated in dorsal midline epidermal cells and not affected in ventral part of tail epidermal cells.

Aniseed comments :
According to the picture, it seems that there is no expression in the CNS and in the palps compared to the control experiment. Moreover, there is still expression in the dorsal midline most posterior tail epidermis.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline posterior tail epidermis - ventral lateral anterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	anterior ventral head epidermis (a7.11 line) - central nervous system - dorsal midline anterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :CAGF9.
Expression pattern : nerve cord, epidermis.
Comment : Epidermal cells expressing this gene are aligned in several lines.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	epidermis - tail nerve cord -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline posterior tail epidermis - ventral head epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Tailbud stage embryo injected with the control LacZ MO, probed for Tox expression.
Original annotation: Injection of control MO into fertilized eggs did not affect the expression.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline posterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Tbx2/3 MO was injected into the left and right posterior animal cells of 8-cell stage embryos. Picture shows embryo at tailbud stage, probed for Tox expression. Original annotation: Tox was greatly reduced in the ventral region, but not in the dorsal region.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Picture shows embryo at tailbud stage, probed for Tox expression, injected with Msx MO. Original annotation: Tox in both the ventral and dorsal regions was lost in Msx morphants, except for a few cells in the posterior-most region.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Embryo with Noggin overexpression under the control of the upstream sequence of Dlx.b, probed for Tox expression.
Original annotation: Tox was lost in the ventral region, but not in the dorsal region.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Ventral and lateral views of an embryo at tailbud stage, probed for Tox expression. Original annotation: strong expression in the ventral tail ectoderm.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline posterior tail epidermis - posterior dorsal midline head epidermis (b8.20 line) - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline head epidermis - dorsal midline posterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 20 (early tailbud II)

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline head epidermis - dorsal midline posterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: 4hr.. Expression pattern: not clear

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	whole embryo -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline posterior tail epidermis - posterior dorsal midline head epidermis (b8.20 line) - tail nerve cord - ventral head epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Aniseed Comment: update of annotation of (Imai et al., 2004), based on (Imai et al., 2006).

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	anterior ventral head epidermis (a7.11 line) - central nervous system - ventral lateral anterior tail epidermis - ventral lateral posterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :CAGF9.
Expression pattern : nerve cord, epidermis.
Comment : Epidermal cells expressing this gene are aligned in several lines.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	epidermis - tail nerve cord -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline head epidermis - dorsal midline posterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline head epidermis - dorsal midline posterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

“Overexpression of Msxb with either pFog or pSoxB2 can activate the other six genes (Nkx-C, Klf1/2/4, Achaete-scute a like-2, Cagf9/tox, Dll-C and Orphan bHLH-1) in the lateral epidermis.”
Embryos are in lateral views with anterior to the left and dorsal to the top.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline head epidermis - dorsal midline posterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline head epidermis - dorsal midline posterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

“Overexpression of Nkx-C with pSoxB2 can activate the other six genes (Nkx-C, Klf1/2/4, Achaete-scute a like-2, Cagf9/tox, Dll-C and Orphan bHLH-1) in the lateral epidermis.
Embryos are in lateral views with anterior to the left and dorsal to the top.

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline head epidermis - dorsal midline posterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 22 (mid tailbud II)

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline head epidermis - dorsal midline posterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: 4hr.. Expression pattern: not clear

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	whole embryo -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	dorsal midline anterior tail epidermis - dorsal midline posterior tail epidermis - posterior dorsal midline head epidermis (b8.20 line) - tail nerve cord - ventral head epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	central nervous system - dorsal midline anterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

“Cagf9/tox was expressed in a portion of the tail nerve cord.”

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 48 (young adult)

Conversion to Aniseed format of Ghost database entry from Ogasawara et al., Dev Genes Evol. 2002; 212:173-185. Original annotation: . Expression pattern: not detected

Stained molecule	KH2012:KH.C3.330 (TOX2; TOX3; TOX4)
Stained region(s)	No expression -