Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Zinc Finger (C2H2)-24.
Expression pattern : No signal was detected.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Zinc Finger (C2H2)-24.
Expression pattern : No distinct zygotic signal was detected.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Zinc Finger (C2H2)-24.
Expression pattern : No distinct zygotic signal was detected.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Zinc Finger (C2H2)-24.
Expression pattern : No distinct zygotic signal was detected.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Zinc Finger (C2H2)-24.
Expression pattern : No distinct zygotic signal was detected.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Zinc Finger (C2H2)-24.
Expression pattern : No distinct zygotic signal was detected.

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Zinc Finger (C2H2)-24.
Expression pattern : nervous system.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Zinc Finger (C2H2)-24.
Expression pattern : epidermis, nervous system.

The arrowheads show the onset of expression at the tip of the tail.
“Klf1/2/4 was expressed in specific domains of the trunk epidermis.”

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: the signal in the trunk is in an O shape. Epidermis along the ventral and dorsal midline.. Expression pattern: Epidermis

Initial tailbud stage wild type embryo probed for ZF(C2H2)-24 expression.

“Klf1/2/4 was expressed in specific domains of the trunk epidermis.”

View of the precursors of the tail epidermis (b4.2 blastomeres) that were isolated at the eight-cell stage and treated with BSA. Explants were probed for KLF1/2/4 expression. Control sibling embryo shows the wild type pattern of the gene. This midline marker of the tail epidermis was not expressed.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Zinc Finger (C2H2)-24.
Expression pattern : epidermis, nervous system.
Comment : brain, epidermis along the dorsal and ventral midline.

Ventral and dorsal view of a tailbud stage wild type embryo probed for ZF(C2H2)-24 expression

Lateral view of a early tailbud stage wild type embryo probed for KLF1/2/4 expression.

Lateral view of an early tailbud stage wild type probed for KLF1/2/4 expression.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.

Original annotation: the signal in the trunk is in an O shape. Epidermis along the ventral and dorsal midline.
Expression pattern: Epidermis

View of the precursors of the tail epidermis (b4.2 blastomeres) that were isolated at the eight-cell stage and treated with BMP4.Explants were probed for KLF1/2/4 expression. Control sibling embryo shows the wild type pattern of the gene and explants treated with BSA. This midline marker of the tail epidermis was expressed throughout these explants.

“Klf1/2/4 was expressed in specific domains of the trunk epidermis.”

Views of early tailbud stage embryos. Reporter expression of the region upstream of Znf(C2H2)-24 was detected in mesenchyme, endodermal strand and part of muscle.

Views of early tailbud satge embryos. Reporter expression of the region upstream of Znf(C2H2) was detected in mesenchyme, muscle and endodermal strand.

Lateral view of an early tailbud stage embryo stained with KLF.

Lateral view of a tailbud stage embryo with anterior to the left stained with Klf1/2/4.

“Klf1/2/4 was expressed in specific domains of the trunk epidermis.”

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: the signal in the trunk is in an O shape. Epidermis along the ventral and dorsal midline.. Expression pattern: Epidermis

Lateral view of a mid tailbud stage embryo stained with KLF. White lines demarcate boundaries between different focal planes. Expression was observed in the epidermal cells.

Picture 1: ventral view of tail epidermis of a mid tailbud stage wild type embryo stained with DAPI

Picture 2: lateral view of a mid tailbud stage wild type embryo probed for KLF1/2/4 expression.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Zinc Finger (C2H2)-24.
Expression pattern : epidermis, nervous system.
Comment : brain, epidermis along the dorsal and ventral midline.

Mid-tailbud stage embryo probed for KLF1/2/4 expression. Whole embryo was treated with BSA.

Lateral view of a mid tailbud stage embryo probed for KLF1/2/4 expression in which Venus under the control of the pFOG driver was expressed. Electroporation of the construct pFOG::Venus has no effect on the midline domain. The expression levels of KLF1/2/4 at mid- to late tailbud stages are lower than at mid-tailbud stages.

Lateral view of a mid tailbud stage wild type embryo , showing KLF1/2/4 expression.

Lateral view of an early tailbud stage wild type probed for KLF1/2/4 expression.

Lateral view of a tailbud stage embryo with anterior to the left stained with Klf1/2/4.
Original annotation: Expression was observed in the dorsal tail epidermis midline.
Aniseed annotation: Expression was also observed in the dorsal midline head epidermis, in the ventral midline anterior tail epidermis and in the ventral midline posterior tail epidermis.

Lateral view with anterior to the left and dorsal to the top of a tailbud stage embryo stained with Klf1/2/4. Expression was observed in the dorsal midline head epidermis, in the ventral midline anterior tail epidermis and in the ventral midline posterior tail epidermis.

“Klf1/2/4 was expressed in specific domains of the trunk epidermis.”

“Klf1/2/4 was expressed in specific domains of the trunk epidermis.”

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: the signal in the trunk is in an O shape. Epidermis along the ventral and dorsal midline.. Expression pattern: Epidermis

Lateral view of an early tailbud stage embryo stained with KLF. Expression was observed in the epidermis along the ventral and dorsal midline

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Zinc Finger (C2H2)-24.
Expression pattern : epidermis, nervous system.
Comment : brain, epidermis along the dorsal and ventral midline.

Lateral view of a late tailbud stage embryo probed for KLF1/2/4 expression in which Venus under the control of the pFOG driver was expressed. The expression levels of KLF1/2/4 at mid- to late tailbud stages are lower than at mid-tailbud stages.

“Klf1/2/4 was expressed in specific domains of the trunk epidermis.”

Views of larvae. Reporter expression of the region upstream of Znf(C2H2)-24 was detected in mesenchyme, endodermal strand and part of muscle.

Views of larvae. Reporter expression of the region upstream of Znf(C2H2) drived by the Fog promoter was detected in mesenchyme, muscle and endodermal strand.