27 results
| Library Name | Percentage | Expression |
|---|---|---|
| Ciona intestinalis whole animal Ciona intestinalis whole animal |
|
0 clone(s) / 0 |
| Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library |
|
2 clone(s) / 5,577 |
| Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult |
|
0 clone(s) / 31,366 |
| Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo |
|
0 clone(s) / 15,579 |
| Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg |
|
0 clone(s) / 31,100 |
| Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva |
|
0 clone(s) / 26,704 |
| Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo |
|
2 clone(s) / 26,680 |
| directional larval cDNA library directional larval cDNA library |
|
0 clone(s) / 39 |
| Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library |
|
0 clone(s) / 0 |
| K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis |
|
0 clone(s) / 5,468 |
| Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library |
|
0 clone(s) / 0 |
| Ciona intestinalis larva Ciona intestinalis larva |
|
0 clone(s) / 0 |
| Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells |
|
0 clone(s) / 29,579 |
| Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle |
|
0 clone(s) / 2,556 |
| Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo |
|
0 clone(s) / 16,939 |
| Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula |
|
0 clone(s) / 25,258 |
| Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad |
|
0 clone(s) / 16,936 |
| Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex |
|
0 clone(s) / 10,463 |
| Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart |
|
0 clone(s) / 13,243 |
| Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland |
|
0 clone(s) / 17,765 |
| Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal |
|
0 clone(s) / 17,872 |
| Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal |
|
4 clone(s) / 107,314 |
| Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal |
|
0 clone(s) / 24,372 |
| Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal |
|
0 clone(s) / 17,126 |
| Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile |
|
0 clone(s) / 3,798 |
| Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx) |
|
0 clone(s) / 2,031 |
| Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) |
|
2 clone(s) / 188,431 |
3 results
OVERVIEW
Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.
Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
|
|
| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 11 (early gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 12 (mid gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 15 (mid neurula) | ||
| C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I) | ||
| C. robusta formely Ciona int. type A, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 11 (early gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 12 (mid gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 15 (mid neurula) | ||
| C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I) | ||
| C. robusta formely Ciona int. type A, Stage 26 (hatching larva) | ||
Ciona robusta adults were obtained from the National Bio-Resource Project for Ciona (NBRP, Japan). 50 unperturbed and Foxd-morphant embryos were collected at the 32-, 64-, and 112-cell stages. RNA was extracted using a Dynabeads mRNA DIRECT Purification Kit (Thermo Fischer Scientific) and libraries were made with an Ion Total RNA-Seq kit ver 2 (Thermo Fischer Scientific). The libraries were sequenced with an Ion PGM instrument (Thermo Fischer Scientific).
| Assay platform | Ion Torrent PGM | |
| Layout | Single-end | |
| Stranded | Yes | |
| Read length | 20-365 | |
| Normalized data types |
|
|
| Wild type | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 6a (early 32-cell) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 10 (112-cell) | ||
| Mutant | ||
| Deregulated molecule(s) | KH2012:KH.C8.890 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 6a (early 32-cell) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 10 (112-cell) | ||
The purpose of this project is to identify genes positively and negatively regulated by signaling molecules that belongs to the TGFbeta superfamily.
| Assay platform | Illumina HiSeq 2500 | |
| Layout | Single-end | |
| Stranded | Yes | |
| Read length | 101 | |
| Normalized data types |
|
|
| Wild type | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
| Mutant 1 | ||
| Deregulated molecule(s) | KH2012:KH.C4.125 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
| Mutant 2 | ||
| Deregulated molecule(s) | KH2012:KH.C2.573 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
121 results
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :FGF8/17/18.
Expression pattern : No signal was detected.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | No expression - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :FGF8/17/18.
Expression pattern : No distinct zygotic signal was detected
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | No expression - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :FGF8/17/18.
Expression pattern : No distinct zygotic signal was detected.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | No expression - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :FGF8/17/18.
Expression pattern : No distinct zygotic signal was detected.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | No expression - |
| Stained molecule | KH2012:KH.C4.84 (CRX; OTX1; OTX2) |
| Stained region(s) | B line - |
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A line - |
Vegetal view of a 44-cell stage wild type embryo probed for Ci-Fgf8/17/18 expression.
Original Annotation :
There is no expression at this stage.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | No expression - |
No picture available in the article.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.6 cell pair - B7.2 cell pair - |
Vegetal view of a 64-cell stage embryo probed for FGF8 after injection with ephrin-Ad MO.
Original annotation: FGF8 displays an almost complete loss of expression.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | No expression - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :FGF8/17/18.
Expression pattern : A7.6
Aniseed annotation :
Weak staining is also observed in A7.1, A7.2 and B7.2 cell pairs
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.6 cell pair - B7.2 cell pair - |
Vegetal view of a 64 cell stage embryo probed for FGF8/17/18 expression.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A7.6 cell pair - |
Vegetal view of a 64-cell stage embryo showing expression of FGF8. Embryos were treated with U0126 at the 16-cell stage (picture and graph) and at the early 32-cell stage, late 32-cell stage and 64-cell stage (graph).
Original annotation:
These embryos often displayed normal expression in A7.6 cell pair but additional patterns were also detected, including expression in A7.6 along with ectopic expression in the endoderm, loss of A7.6 expression along with gain of expression in the endoderm, or complete loss of expression.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A7.6 cell pair - |
Original annotation :
Gene name :FGF8/17/18.
Expression pattern : A7.6
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A8.11 cell pair - A8.12 cell pair - |
Vegetal view of a 110-cell stage embryo probed for FGF8/17/18 expression following egg micro-injection of a Neurogenin MO. FGF8/17/18 was down-regulated in TLCs.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | No expression - |
Vegetal view of a 110-cell stage embryo probed for FGF8/17/18 expression following egg micro-injection of a FoxD-a/b MO. FGF8/17/18 was down-regulated in TLCs.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | No expression - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :FGF8/17/18.
Expression pattern : A7.6
Aniseed annotation :
Weak staining is also observed in A7.1 and A.2 cell pairs
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A8.11 cell pair - A8.12 cell pair - |
Vegetal view of a 110 cell stage embryo probed for FGF8/17/18 expression.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A8.11 cell pair - A8.12 cell pair - |
Vegetal view of a 110-cell stage embryo showing expression of FGF8 in A7.6 cell pair.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A8.11 cell pair - A8.12 cell pair - |
Vegetal view of a 110-cell stage embryo showing expression of FGF8. Embryos were treated with U0126 at the late 32-cell stage.
Original annotation: A7.6 group genes are ectopically expressed in the anterior endoderm blastomeres upon MEK inhibition, whereas A7.6 expression is unaffected.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A8.11 cell pair - A8.12 cell pair - |
Vegetal views of embryos injected at the 1-cell stage with torso-FGFR mRNA and probed for FGF8.
Original annotation:
In situ hybridization assays revealed consistent reductions in FGF8 expression in A7.6.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A8.11 cell pair - A8.12 cell pair - |
Vegetal view of a 110-cell stage embryo probed for FGF8 and co-injected with ephrin-Ad MO and MEK inhibitor U0126 at the 32-cell stage.
Original annotation:
FGF8 is expressed in both the A7.6 blastomere and the anterior endoderm.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A8.11 cell pair - A8.12 cell pair - |
Vegetal view of a 110-cell stage embryo probed for FGF8 following injection of FoxD::ephrin-Ad. This construct was used to drive Ci-ephrin-Ad throughout the vegetal hemisphere at the 16-cell stage (thanks to FoxD enhancer).
Original annotation:
FGF8 expression is observed in A7.6 blastomeres and in anterior endoderm (arrows).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A8.11 cell pair - A8.12 cell pair - |
Vegetal view of an early gastrula stage wild type embryo probed for Ci-Fgf8/17/18 expression.
Original Annotation :
Ci-Fgf8/17/18 expression was detected in A7.6 cell pair (arrowheads).
Aniseed Annotation :
According to the Aniseed anatomical dictionary, A7.6 cells are already divided in A8.11 and A8.12 cells.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A8.11 cell pair - A8.12 cell pair - |
Vegetal view with anterior to the top and posteror view of a mid-gastrula stage embryo probed for Ci-fgf8/17/18 expression. DAPI staining in blue.
Original annotation: Expression was observed in a small population (2 to 4 cells) of epidermal cells at the tip of the extending tail (indicated by red arrowheads).
Aniseed annotation: Expression is also observed in the A9.30 cell pair.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - dorsal posterior tail epidermis - |
Vegetal view of a gastrula stage wild type embryo probed for Ci-FGF8/17/18 expression.
Original Annotation :
Ci-FGF8/17/18 expression was detected in A9.30 cell pair (red arrowheads).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Vegetal view of a gastrula stage embryo probed for Ci-FGF8/17/18 expression, following the injection of Neurogenin-MO.
Original Annotation :
Ci-FGF8/17/18 expression became ectopic following the disruption of neurogenin signaling and was detected in A9.30 and A9.32 cell pairs (red and white arrowheads respectively).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - A9.32 cell pair - |
Vegetal view of a gastrula stage embryo probed for Ci-FGF8/17/18 expression, following the injection of Snail-MO.
Original Annotation :
Ci-FGF8/17/18 expression, following the disruption of Ci-snail signaling, was detected only in A9.30 cells (red arrowheads).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Vegetal view of a late gastrula wild type embryo probed for Ci-FGF8/17/18 expression.
Original Annotation :
Ci-FGF8/17/18 expression was detected in A9.30 cell pair.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Vegetal view of a late gastrula embryo probed for Ci-FGF8/17/18 expression, following the injection of Neurogenin-MO.
Original annotation:
Ci-FGF8/17/18 expression became ectopic in A9.32 cells (black arrow).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - A9.32 cell pair - |
Vegetal view of a late gastrula stage embryo probed for Ci-FGF8/17/18 expression, following the injction of Delta-like-MO.
Original annotation:
Ci-FGF8/17/18 expression became ectopic in A9.32 following the disruption of Delta-like signalling.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - A9.32 cell pair - |
Vegetal view of a late gastrula stage embryo probed for Ci-FGF8/17/18 expression following the injction of Otx-MO.
Original annotation:
Ci-FGF8/17/18 expression was not modified by the injection of Otx-MO.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Vegetal view of a late gastrula embryo probed for Ci-FGF8/17/18 expression, following the injection of Snail-MO.
Original annotation:
Ci-FGF8/17/18 expression became ectopic in A9.32 cell pair (black arrow).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - A9.32 cell pair - |
Vegetal view of a late gastrula stage embryo probed for Ci-FGF8/17/18 expression following the injection of Snail MO (snail-2nd MO (Imai 2009)). Note that in the article, two MO against snail were used (in Aniseed : snail-MO (Imai 2009) and snail-2nd MO (Imai 2009)).
Original annotation:
Ci-FGF8/17/18 expression became ectopic in A9.32 cell pair (black arrow).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - A9.32 cell pair - |
Neural plate view of a Mid gastrula stage wild type embryo probed for Ci-FGF8/17/18 expression.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Neural plate view of a Late gastrula stage embryo probed for Ci-FGF8/17/18 expression in which Nodal signalling has been blocked by injection with Nodal MO. Expression in A9.30 was lost. Control sibling embryo shows the wild type pattern of the gene.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | No expression - |
Neural plate view of a Late gastrula stage embryo probed for Ci-FGF8/17/18 expression in which Nodal signalling has been blocked by tretament with SB431642 type I receptor inhibitor. The expression in A9.30 was lost. Control sibling embryo shows the wild type pattern of the gene.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | No expression - |
Vegetal view of a wild type embryo probed for Ci-FGF8/17/18 expression.
Original Annotation : Ci-FGF8/17/18 expression was detected in neural plate, in A9.30 cell pair.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Vegetal view of an embryo probed for Ci-FGF8/17/18 expression following the injection of DAPT.
Graph shows the percentage of embryo with ectopic, strong or weak Ci-FGF8/17/18 expression following the injection of DAPT. 'Weak' means at least one cell with downregulated expression, 'strong' means at least one A-line precursor cell with strong expression, sometimes there is ectopic expression.
Original Annotation : In the majority of embryos Ci-FGF8/17/18 expression was not affected by the inhibition of Delta2/Notch signaling due to DAPT.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Vegetal view of embryo probed for Ci-FGF8/17/18 expression following the electroportation of a promoter FOG::Delta2.
Graph shows the percentage of embryo with ectopic, strong or weak Ci-FGF8/17/18 expression following the electroporation of FOG::Delta2. 'Weak' means at least one cell with downregulated expression, 'strong' means at least one A-line precursor cell with strong expression, sometimes there is ectopic expression.
Original Annotation : in the majority of embryos Ci-FGF8/17/18 expression was not affected by the electroporation of FOG::Delta2 construct. But in 49% of embryos Ci-FGF8/17/18 was expressed on one side only (weak expression in graph).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Vegetal view of embryo probed for Ci-FGF8/17/18 expression following the injection of Delta2-MO.
Graph shows the percentage of embryo with ectopic, strong or weak Ci-FGF8/17/18 expression following the injection of Delta2-MO.'Weak' means at least one cell with downregulated expression, 'strong' means at least one A-line precursor cell with strong expression, sometimes there is ectopic expression.
Original Annotation : in the majority of embryos Ci-FGF8/17/18 expression was not affected by the inhibition of Delta2/Notch signaling due to Delta2-MO. Occasionally, following disruption of Delta2/Notch signaling ectopic expression was seen for this gene.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Graph shows the percentage of mid gastrula embryo with ectopic, strong or weak Ci-FGF8/17/18 expression following the injection of dnDel2, a dominant negative of Delta2.'Weak' means at least one cell with downregulated expression, and 'strong' means at least one A-line precursor cell with strong expression.
Original Annotation : In the majority of embryos Ci-FGF8/17/18 expression was not affected by the inhibition of Delta2/Notch signaling due to dnDel2. But there is also a important part of embryos with ectopic expression following disruption of Delta2/Notch signaling.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Vegetal view of a wild type embryo probed for Ci-FGF8/17/18 expression.
Original Annotation : Ci-FGF8/17/18 expression was detected in neural plate, in A9.30 cell pair.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Vegetal views of an embryo probed for Ci-FGF8/17/18 expression, following the treatment of U0126. In the last picture, nuclei are labeled with Hoechst (white) to allow easy identification of individual cells.
Original Annotation : Following the inhibition of MEK/ERK activity, Ci-FGF8/17/18 was expressed ectopically in A9.29 cell pair.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.29 cell pair - A9.30 cell pair - |
Vegetal view of a late gastrula stage wild type embryo probed for Ci-FGF8/17/18 expression.
Original Annotation :
Ci-FGF8/17/18 expression was detected in A9.30 cell pair (arrowheads).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Vegetative view of a mid gastrula. FGF8/17/18 is expressed in A9.30 blastomer pair (row II of neural plate)
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Vegetative view of a mid gastrula probed for Fgf8/17/18 expression, following injection of Snail morpholino (Snail-MO (Imai's 25mer MO)).
Original annotation : Expression of Fgf8/17/18 was largely unaffected by Snail knockdown, ectopic expression of Fgf8/17/18 in row I/column 4 was rarelly observed (red arrowhead).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - A9.32 cell pair - |
Vegetative view of a mid gastrula probed for Fgf8/17/18 expression, following injection of Snail morpholino (Snail-MO_2 (Hudson 2015)).
Original annotation : Expression of Fgf8/17/18 was largely unaffected by Snail knockdown.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Vegetative view of a mid gastrula. Fgf8/17/18 is expressed in A9.30 blastomer pair (row II of neural plate)
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Vegetative view of a mid gastrula probed for Fgf8/17/18 expression, where Snail is overexpressed in medial lateral neural plate (electroporation of pETR>Snail). Signal is detected in A9.30 (row II of neural plate), as in WT embryos.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Vegetative view of a mid gastrula probed for Fgf8/17/18 expression and treated with SB431542 (Nodal inhibitor) until early gastrula stage. No signal was detected.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | No expression - |
Vegetative view of a mid gastrula probed for Fgf8/17/18 expression and treated with SB431542 (Nodal inhibitor) until early gastrula stage, where Snail is overexpressed in medial lateral neural plate (electroporation of pETR>Snail). No signal is detected.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | No expression - |
Vegetative view of a mid gastrula probed for Fgf8/17/18 expression, where Nodal is overexpressed in animal territories (electroporation of pFOG>Nodal).
Original annotation : Nodal overexpression was able to induce ectopic expression of Fgf8/17/18 in its correct row of cells, row II.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | row I neural plate - |
Dorsal view of a WT Ciona robusta late gastrula embryo probed for Fgf8/18/19 expression. Cell nuclei are counterstained by Hoechst stain.
Original annotation: A9.30 blastomeres are labeled by for Fgf8/18/19 mRNA in red. The outlined cells are represented in the diagram, representing the right half of the bilaterally symmetric posterior neural plate.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Neural plate view of a Late gastrula stage wild type embryo probed for Ci-FGF8/17/18 expression.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :FGF8/17/18.
Expression pattern : mesenchyme, nervous system.
Comment : Nervous system: A9.30
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Aniseed Comment: Vegetal view of a late gastrula stage wild type embryo probed for Fgf8/17/18 expression. Expression was detected in A9.30 cell pair.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A9.30 cell pair - |
Vegetal view of a late gastrula stage embryo probed for Fgf8/17/18 expression following egg micro-injection of a FoxB MO. FGF8/17/18 was ectopically expressed in medial part of A-line nerve cord cells.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | A line neural plate - |
Lateral view of a mid neurula wild type embryo probed for Ci-Fgf8/17/18 expression.
Original annotation:
In normal development, Ci-Fgf8/17/18 transcripts are detectable by WISH at the posterior end of the embryo from the neurula stage onwards.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | posterior tail epidermis - |
Lateral view of a mid neurula embryos probed for Ci-Fgf8/17/18 expression following the injection of Ci-Hox12-MO.
Original annotation:
The frequency of Ci-Fgf8/17/18-positive embryos, which were indistinguishable from normal embryos, was 100% at the neurula stage.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | posterior tail epidermis - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :FGF8/17/18.
Expression pattern : mesenchyme, epidermis
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | tail epidermis - |
Neurula stage wild type embryo probed for Ci-FGF8/17/18 expression.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | posterior tail epidermis - |
Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Epidermis
BE CAREFUL: seems to be expression pattern of FGF3 and not of FGF8... To be checked.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | epidermis - |
Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Epidermis
BE CAREFUL: seems to be expression pattern of FGF3 and not of FGF8... To be checked.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | epidermis - |
Lateral view with anterior to the left and posteror view of an early tailbud stage embryo probed for Ci-fgf8/17/18 expression. DAPI staining in blue.
Original annotation: Expression was observed in a small population (2 to 4 cells) of epidermal cells at the tip of the extending tail (indicated by red arrowheads).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | posterior tail epidermis - |
Lateral view with anterior to the left of a mid-tailbud stage embryo stained probed for fgf8/17/18 expression.
Original annotation: Expression was observed in the 2-4 cells at the very tip of the rail.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | mesenchyme (B8.5 & B7.7 lines) - posterior tail epidermis - |
Lateral view with anterior to the left of a mid-tailbud stage embryo probed for fgf8/17/18 expression after retinoic acide treatment (1.5μM) at the late gastrula stage.
Original annotation: Expression was observed in the 2-4 cells at the very tip of the rail.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | mesenchyme (B8.5 & B7.7 lines) - posterior tail epidermis - |
Lateral view with anterior to the left of a mid-tailbud stage embryo probed for fgf8/17/18 expression after DEAB treatment (150μM) at the late gastrula stage.
Original annotation: Expression was observed in the 2-4 cells at the very tip of the rail.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | posterior tail epidermis - |
Lateral view with anterior to the left of a mid-tailbud stage embryo stained with fgf8/17/18 after bFGF treatment (100ng/ml) at the late gastrula stage.
Original annotation: Expression was observed in the 2-4 cells at the very tip of the rail.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | posterior tail epidermis - |
Lateral view with anterior to the left of a mid-tailbud stage embryo stained with fgf8/17/18 after injection of a dominant-negative forme of the Ciona FGF receptor (Ci-FGFR) at the late gastrula stage.
Original annotation: Expression was lost in the 2-4 cells at the very tip of the rail.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | mesenchyme (B8.5 & B7.7 lines) - |
Lateral view with anterior to the left of a mid-tailbud stage embryo probed for fgf8/17/18 expression after U0126 treatment (10μM) at the late gastrula stage.
Original annotation: Expression was lost in the 2-4 cells at the very tip of the rail.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | No expression - |
Lateral view of an early tailbud wild type embryo probed for Ci-Fgf8/17/18 expression.
Original annotation:
In normal development, Ci-Fgf8/17/18 transcripts are detectable by WISH at the posterior end of the embryo from the neurula stage onwards.
Aniseed annotation:
Weak expression is observed in mesenchyme.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | posterior tail epidermis - |
Lateral view of an early tailbud embryo probed for Ci-Fgf8/17/18 expression following the injection of Ci-Hox12-MO.
Original annotation:
The frequency of Ci-Fgf8/17/18-positive embryos, which were indistinguishable from normal embryos, was 50% at the early tailbud stage.
Aniseed annotation:
Weak expression is observed in mesenchyme.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | posterior tail epidermis - |
Lateral view of an early tailbud stage embryo probed for En and Fgf8/17/18 expression.
Initial annotation :
Ci-Fgf8/17/18 is expressed in the same region as the posterior Ci-En expression domain.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | visceral ganglion - |
Lateral and dorsal views of an early tailbud stage embryo probed for Fgf8/17/18 expression.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | anterior sensory vesicle - posterior sensory vesicle - tail epidermis - visceral ganglion - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :FGF8/17/18.
Expression pattern : mesenchyme, epidermis.
Comment : epidermis: tip of the tail
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | tail epidermis - |
Dorsal view of an early tailbud stage embryo probed for Ci-Fgf8/17/18 expression.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | trunk lateral cells (A7.6 line) - |
Vegetal view of an early tailbud stage embryo probed for Ci-FGF8/17/18 expression, following the injection of FGF8/17/18-MO.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.
Original Annotation :
Ectopic expression was observed in the visceral ganglion (black arrow).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | trunk lateral cells (A7.6 line) - visceral ganglion - |
Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Epidermis
BE CAREFUL: seems to be expression pattern of FGF3 and not of FGF8... To be checked.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | epidermis - |
The central nervous system of a mid tailbud wild type embryo co-electroporated with FGF8/17/18>RFP (red) and AchTP>GFP (green).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | posterior sensory vesicle - visceral ganglion - |
The central nervous system of a mid tailbud stage wild type embryo co-electroporated with FGF8/17/18>RFP (red) and FoxB>GFP (green).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | posterior sensory vesicle - visceral ganglion - |
Comparison of Ci-FGF8/17/18 (blue stain) and Ci-Dmbx (red stain) at mid tailbud stage (lateral view) showing co-localised expression (arrowhead). Ci-Dmbx staining is coincident with Ci-FGF8/17/18 expression in the visceral ganglion. Additional expression of Ci-FGF8/17/18 is detected in the trunk-lateral cells and at the tip of the tail after prolonged staining (data not shown).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | trunk lateral cells (A7.6 line) - visceral ganglion - |
Lateral view of a mid tailbud wild type embryo probed for Ci-Fgf8/17/18 expression.
Original annotation:
We identified Ci-Fgf8/17/18 as the genes expressed in the tail tip epidermis in normal mid-tailbud embryos.
Aniseed annotation:
Expression observed also in the mesenchyme.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | posterior tail epidermis - |
Lateral view of a mid tailbud embryo probed for Ci-Fgf8/17/18 expression following the injection of Ci-Hox12-MO.
Original annotation:
Expression of Ci-Fgf8/17/18 in the tail tip epidermis was extensively reduced in Hox12MO-injected embryos, whereas expression outside of the tail tip was unaffected.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) |
Lateral view of a mid tailbud embryo probed for Ci-Fgf8/17/18 expression following the injection of Ci-Hox12-MO and Ci-Hox12 mRNA.
Original annotation:
Expression of Ci-Fgf8/17/18 at the tail tip was recovered.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | posterior tail epidermis - |
Lateral view of a mid tailbud embryo probed for Ci-Fgf8/17/18 expression following the injection of Wnt5-MO in the b4.2 cells at 8-cell stage.
Original annotation:
Ci-Fgf8/17/18 expression in the tail tip epidermis was unaffected in embryos injected into the epidermal lineage with Wnt5MO.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | posterior tail epidermis - |
Dorsal views of mid tailbud stage embryo expressing Fgf8/17/18. The upper panel shows WISH signals, the middle DAPI signals and the lower the merged images.
Arrowheads indicate nuclei of the cells that express Fgf8/17/18. Open arrows indicate the constrictions in the row of nuclei of the lateral cells. Red dots indicate nuclei of the landmark cells.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | lateral VG ependymal precursors - |
Two-color-fluorescence WISH was performed at the mid tailbud stage. Fgf8/17/18 expression is shown in red, Dmbx in green.
Original annotation:
Ci-Fgf8/17/18 is expressed in the mesenchyme and part of the visceral ganglion. The Ci-Dmbx domain did not overlap with the Ci-FGF8/17/18 domain.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | visceral ganglion - |
Two-color-fluorescence WISH was performed at the mid tailbud stage. Fgf8/17/18 expression is shown in green, SoxB1 in red.
Original annotation:
Ci-Fgf8/17/18 is expressed in the mesenchyme and in the visceral ganglion. Ci-Fgf8/17/18 is expressed just behind the second domain of Ci-SoxB1.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | visceral ganglion - |
Two-color-fluorescence WISH was performed at the mid tailbud stage. Fgf8/17/18 expression is shown in green, vACHTP in red.
Ci-Fgf8/17/18 is expressed in the mesenchyme and in part of the visceral ganglion.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | visceral ganglion - |
Two-color fluorescence WISH was performed at the mid tailbud stage. Fgf8/17/18 expression is shown in green, Lhx3 in red.
Ci-Fgf8/17/18 is expressed in the mesenchyme and in part of the visceral ganglion.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | visceral ganglion - |
Two-color fluorescence WISH was performed at the mid tailbud stage. Fgf8/17/18 expression is shown in red, En in green.
Original annotation:
Ci-Fgf8/17/18 is expressed in the mesenchyme and in part of the visceral ganglion. Expression of Ci-Fgf8/17/18 was detected in a small region of the CNS, which overlaps with posterior part of the third domain of Ci-En.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | visceral ganglion - |
Two-color fluorescence WISH was performed at the mid tailbud stage. Fgf8/17/18 expression is shown in red.
Original annotation:
Ci-Fgf8/17/18 is expressed in the mesenchyme and part of the visceral ganglion. Expression of Ci-Fgf8/17/18 was detected in a small region of the CNS, which overlaps with posterior part of the first domain of Ci-Hox1.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | visceral ganglion - |
Dorsal view of a mid tailbud stage embryo probed for Ci-Fgf8/17/18 expression.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.
Original annotation:
Expression in the visceral ganglion was localized in A13.473 and A12.239 cell pairs.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | trunk lateral cells (A7.6 line) - visceral ganglion - |
Lateral view with anterior to the left of a mid-tailbud stage embryo stained with Ci-fgf8/17/18.
Original annotation: Expression was observed in a small population (2 to 4 cells) of epidermal cells at the tip of the extending tail (indicated by red arrowheads).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | posterior tail epidermis - |
The central nervous system of a late tailbud stage wild type embryo co-electroporated with FGF8/17/18>RFP (red) and AchTP>GFP (green). Arrowheads indicate motoneurons.
Original Annotation :
Ci-FGF8/17/18 was detected in the central nervous system more precisely in the lateral visceral ganglion ependymal cells and motoneurons in the post sensory vesicle.
The arrowheads indicate motoneurons.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | posterior sensory vesicle - visceral ganglion - |
Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Mesenchyme
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) |
Dorsal views of late tailbud stage embryo expressing Fgf8/17/18. The upper panel shows WISH signals, the middle DAPI signals and the lower the merged images.
Arrowheads indicate nuclei of the cells that express Fgf8/17/18. Open arrows indicate the constrictions in the row of nuclei of the lateral cells. Red dots indicate nuclei of the landmark cells.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | lateral VG ependymal cells - |
Two-color fluorescence WISH was performed at the late tailbud stage. Fgf8/17/18 expression is shown in red, En in green.
Original annotation:
Ci-Fgf8/17/18 is expressed in part of the visceral ganglion. Expression of Ci-En was detected in three domains. The posterior-most part of the first domainof Ci-Fgf8/17/18 expression domain do not overlap with the third domain of Ci-En.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | visceral ganglion - |
Two-color-fluorescence WISH was performed at the late tailbud stage. Fgf8/17/18 expression is shown in green, SoxB1 in red.
Original annotation:
Ci-Fgf8/17/18 is expressed in the visceral ganglion. Signal for Ci-Fgf8/17/18 is located between the third and fourth domains of Ci-SoxB1.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | visceral ganglion - |
Two-color-fluorescence WISH was performed at the late tailbud stage. Fgf8/17/18 expression is shown in green.
Original annotation:
Ci-Fgf8/17/18 is expressed in part of the visceral ganglion. Expression of Ci-Lhx3 in the posterior CNS is detected in two domains with a narrow gap between them. The first domain of Ci-Lhx3 coincides with the Fgf8/17/18 domain.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | visceral ganglion - |
Two-color fluoresce. Fgf8/17/18 expression is shown in green, vACHTP in red. Ci-Fgf8/17/18 is expressed in part of the visceral ganglion.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | visceral ganglion - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :FGF8/17/18.
Expression pattern : mesenchyme, nervous system, epidermis.
Comment : epidermis: tip of the tail, nervous system: a putative mid/hindbrain boundary
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | neck - tail epidermis - |
Lateral view of the head of a WT Ciona robusta stage E65-E70 tailbud embryo [~15 hpf at 16°C], with descendants of A9.30 labeled by mCherry driven by the Fgf8/17/18 enhancer in red. Labelled blastomeres are A12.240, A12.239, A12.238, A12.237, A11.118 and A11.117 pairs.
Cell membranes are counterstained with phallicidin:BODIPY-FL.
Original annotation: Outlined are the cells represented in the diagram on the right, representing the posterior neural tube, comprising four rows of cells and derived from the posterior neural plate. The dorsal row of cells is slightly lifted to reveal descendants of both A9.30 blastomeres (red) on either side of the embryo.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | lateral VG ependymal cells - visceral ganglion - |
Lateral view of a WT Ciona robusta late tailbud embryo electroporated with Fgf8/17/18>nlsLacZ (red) and
probed for Islet.
C' is a higher magnification of C.
Original annotation:A9.30 lineage is labeled by electroporation with Fgf8/17/18>lacZ (red).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | visceral ganglion - |
Lateral view of the visceral ganglion of a WT Ciona robusta late tailbud embryo co-electroporated with Dmbx>unc76::GFP (green) and Fgf8/17/18>unc76::mCherry (red) reporter constructs.
Original annotation:A9.30 lineage is
labeled by electroporation of Fgf8/17/18>mCherry.
Colocalization of GFP and mCherry is indicated in
yellow and corresponds to Dmbx>GFP activity in A12.239.
A fusion of 3.5 kb of Dmbx upstream DNA with unc-76-tagged enhanced green fluorescent protein (GFP) recapitulated strong expression in A12.239, with slight expression in its sister cell, A12.240
| Stained molecule | KH2012:KH.C1.1212 (DMBX1; DRGX; PITX3) |
| Stained region(s) | visceral ganglion - |
Lateral view of the visceral ganglion of a WT Ciona robusta late tailbud embryo co-electroporated with Dmbx>unc76::GFP (green) and Fgf8/17/18>unc76::mCherry (red) reporter constructs.
Original annotation:A9.30 lineage is
labeled by electroporation of Fgf8/17/18>mCherry.
Colocalization of GFP and mCherry is indicated in
yellow and corresponds to Dmbx>GFP activity in A12.239.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | lateral VG ependymal cells - visceral ganglion - |
Lateral view of the visceral ganglion of a WT Ciona robusta late tailbud embryo co-electroporated with Vsx>unc76::GFP (green) and Fgf8/17/18>unc76::mCherry (red) reporter constructs.
Original annotation:A9.30 lineage is
labeled by electroporation of Fgf8/17/18>mCherry.
Colocalization of GFP and mCherry is indicated in
yellow and corresponds to Vsx>GFP activity in A11.117. Vsx>GFP is not seen in A13.474, as this cell has not yet been born.
A fusion of a DNA sequence spanning the entire Vsx transcribed region with unc-76-tagged enhanced green fluorescent protein (GFP) recapitulated expression in A11.117 and A13.474.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | visceral ganglion - |
Lateral view of the visceral ganglion of a WT Ciona robusta late tailbud embryo co-electroporated with Vsx>unc76::GFP (green) and Fgf8/17/18>unc76::mCherry (red) reporter constructs.
Original annotation:A9.30 lineage is
labeled by electroporation of Fgf8/17/18>mCherry.
Colocalization of GFP and mCherry is indicated in
yellow and corresponds to Vsx>GFP activity in A11.117. Vsx>GFP is not seen in A13.474, as this cell has not yet been born.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | lateral VG ependymal cells - visceral ganglion - |
Lateral view of a WT Ciona robusta late tailbud embryo electroporated with Fgf8/17/18>nlsLacZ (red) and probed for Dmbx expression.
A' is a higher magnification of A.
Original annotation: A9.30 lineage is labeled by electroporation with Fgf8/17/18>lacZ (red).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | lateral VG ependymal cells - visceral ganglion - |
Lateral view of a WT Ciona robusta late tailbud embryo electroporated with Fgf8/17/18>nlsLacZ (red) and probed for Vsx expression (green).
B' is a higher magnification of B.
Original annotation: A9.30 lineage is labeled by electroporation with Fgf8/17/18>lacZ (red).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | lateral VG ependymal cells - visceral ganglion - |
Lateral view of the visceral ganglion of a WT Ciona robusta late tailbud embryo co-electroporated with Islet>unc76::GFP (green) and Fgf8/17/18>unc76::mCherry (red) reporter constructs.
Original annotation:A9.30 lineage is
labeled by electroporation of Fgf8/17/18>mCherry.
The upstream enhancer of Islet drives reporter gene expression in A10.64 (motor neuron 2 precursor).
| Stained molecule | KH2012:KH.L152.2 (ISL1; ISL2; LHX9) |
| Stained region(s) | visceral ganglion - |
Lateral view of the visceral ganglion of a WT Ciona robusta late tailbud embryo co-electroporated with Islet>unc76::GFP (green) and Fgf8/17/18>unc76::mCherry (red) reporter constructs.
Original annotation: A9.30 lineage is
labeled by electroporation of Fgf8/17/18>mCherry.
The upstream enhancer of Islet drives reporter gene expression in A10.64 (motor neuron 2 precursor).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | lateral VG ependymal cells - visceral ganglion - |
Lateral view of the neck region of a WT Ciona robusta late tailbud embryo co-electroporated with COE>unc76::GFP (green) and Fgf8/17/18>unc76::mCherry (red) reporter constructs.
Original annotation: A 2.6 kb genomic DNA segment located upstream of the Coe gene directs expression in all of the differentiating visceral ganglion neural precursors (green).
(B) A9.30 lineage is marked by FGF8/17/18>mCherry (red).
(C) Merged view of A and B. COE>GFP marks both A12.240 and A12.239 (anterior-most cells), owing to transient expression in mother cell A11.120. Endogenous Coe expression becomes restricted to A12.239 and excluded from A12.240 starting at this time (data not shown).
| Stained molecule | KH2012:KH.L24.10 (EBF1; EBF2; EBF3) |
| Stained region(s) | lateral VG ependymal cells - |
Lateral view of the neck region of a WT Ciona robusta late tailbud embryo co-electroporated with COE>unc76::GFP (green) and Fgf8/17/18>unc76::mCherry (red) reporter constructs.
Original annotation:A9.30 lineage is marked by FGF8/17/18>mCherry (red).
(A) Coe enhancer directs GFP expression in all of the differentiating visceral ganglion neural precursors (green).
(C) Merged view of A and B.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | lateral VG ependymal cells - visceral ganglion - |
Dorsal view of the visceral ganglion of a WT Ciona robusta late tailbud embryo co-electroporated with Vsx>unc76::GFP (green) and Fgf8/17/18>H2B::mCherry (red) reporter constructs.
Original annotation: At stage E75 (15.5 hpf),
Vsx>GFP (green) is normally visible only in A11.117.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | lateral VG ependymal cells - |
Dorsal view of the visceral ganglion of a WT Ciona robusta late tailbud embryo co-electroporated with Vsx>unc76::GFP (green) and Fgf8/17/18>H2B::mCherry (red) reporter constructs.
Fgf8/17/18 enhancer drives mCherry expression in the nuclei of A9.30 lineage cells and some mesenchyme cells.
Original annotation: Asterisks denote mesenchyme cells expressing Fgf8/17/18.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | lateral VG ependymal cells - mesenchyme (B8.5 & B7.7 lines) - |
Dorsal view of the visceral ganglion of a Ciona robusta late tailbud embryo co-electroporated with Vsx>unc76::GFP (green) and Fgf8/17/18>H2B::mCherry (red) reporter constructs and Fgf8/17/18>dnFGFR overexpression construct.
Vsx enhancer drives GFP expression in all the A9.30 lineage cells.
Original annotation: )The Fgf8/17/18 enhancer was used to drive expression of a truncated, dominant-negative FGF receptor [dnFGFR (Davidson et al., 2006)] in A9.30. Lineage-specific perturbation of signaling downstream of FGFR resulted in ectopic A11.117-like cells. In 90% (n=90) of electroporated embryos, all A9.30 descendants express Vsx>GFP.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | lateral VG ependymal cells - visceral ganglion - |
Dorsal view of the visceral ganglion of a Ciona robusta late tailbud embryo co-electroporated with Vsx>unc76::GFP (green) and Fgf8/17/18>H2B::mCherry (red) reporter constructs and Fgf8/17/18>dnFGFR overexpression construct.
Fgf8/17/18 enhancer drives mCherry expression in the nuclei of the A9.30 lineage cells.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | lateral VG ependymal cells - visceral ganglion - |
Lateral view of a WT Ciona robusta larva co-electroporated with Fgf8/17/18>unc76::GFP (green) and COE>LacZ (not observed, control experiment).
Fgf8/17/18 directs GFP expression in the A9.30 lineage, id est motor neurons and visceral ganglion.
Original annotation: Control larva showing frondose endplates from A9.30 lineage labeled by Fgf8/17/18>GFP electroporation.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | motor neurones - visceral ganglion - |
Dorso-lateral views (different focal planes) of Ciona robusta larva co-electroporated with Fgf8/17/18>unc76::GFP (green) and COE>Dmbx overexpression construct.
Fgf8/17/18 directs GFP expression in the A9.30 lineage, id est motor neurons and visceral ganglion.
Original annotation: Axons from A9.30 descendants
in the visceral ganglion project down the tail but do not form motor endplates (inset). The second image shows multiple axon growth cones labeled by Fgf8/17/18>GFP extending down the tail. This demonstrates that lack of endplates seen upon Dmbx overexpression is not due to failure of neural differentiation in general.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | motor neurones - visceral ganglion - |
Lateral view of the tail of a Ciona robusta larva co-electroporated with Fgf8/17/18>unc76::GFP (green) and COE>Vsx overexpression construct.
Fgf8/17/18 directs GFP expression in the A9.30 lineage, id est motor neurons and visceral ganglion.
Original annotation: Axons from A9.30 descendants
in the visceral ganglion project down the tail but do not form motor endplates.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | motor neurones - visceral ganglion - |
Lateral view of the tail of a WT Ciona robusta larva co-electroporated with Fgf8/17/18>unc76::GFP (green) and COE>Islet overexpression construct.
Fgf8/17/18 directs GFP expression in the A9.30 lineage, id est motor neurons and visceral ganglion.
Original annotation: Electroporation of Coe>Islet did not have a visible effect on motor endplate formation. This observation is not surprising as Islet is transiently expressed in A11.118 in wild-type larvae.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | motor neurones - visceral ganglion - |
Lateral view of the tail of a WT Ciona robusta larva co-electroporated with Fgf8/17/18>unc76::GFP (green) and COE>Nk6 overexpression construct.
Fgf8/17/18 directs GFP expression in the A9.30 lineage, id est motor neurons and visceral ganglion.
Original annotation: Electroporation of Coe>Nk6 did not have a visible effect on motor endplate formation. This observation is not surprising as Nk6 is transiently expressed in A11.118 in wild-type larvae.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | motor neurones - visceral ganglion - |
Dorsal view of the visceral ganglion of a WT Ciona robusta larva co-electroporated with Dmbx>unc76::GFP (green) and Fgf8/17/18>H2B::mCherry (red) reporter constructs.
Dmbx enhancer drives GFP expression in A12.239 motor neuron precursor.
| Stained molecule | KH2012:KH.C1.1212 (DMBX1; DRGX; PITX3) |
| Stained region(s) | lateral VG ependymal cells - |
Dorsal view of the visceral ganglion of a WT Ciona robusta larva co-electroporated with Dmbx>unc76::GFP (green) and Fgf8/17/18>H2B::mCherry (red) reporter constructs.
Fgf8/17/18 enhancer drives mCherry expression in the nuclei of the A9.30 lineage cells.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | lateral VG ependymal cells - visceral ganglion - |
Dorsal view of the visceral ganglion of a Ciona robusta larva co-electroporated with Dmbx>unc76::GFP (green) and Fgf8/17/18>H2B::mCherry (red) reporter constructs and Fgf8/17/18>Su(H)DBM overexpression construct.
Original annotation:Upon inhibition
of Notch signaling by co-electroporation with Fgf8/17/18>Su(H)DBM, Dmbx>GFP is seen to be expressed in two A9.30 descendants, instead of just A12.239.
| Stained molecule | KH2012:KH.C1.1212 (DMBX1; DRGX; PITX3) |
| Stained region(s) | lateral VG ependymal cells - |
Dorsal view of the visceral ganglion of a Ciona robusta larva co-electroporated with Dmbx>unc76::GFP (green) and Fgf8/17/18>H2B::mCherry (red) reporter constructs and Fgf8/17/18>Su(H)DBM overexpression construct.
Nuclei from A9.30 lineage are labeled by electroporation with Fgf8/17/18>lacZ (red).
The asterisk denotes a mesenchyme cell expressing
Fgf8/17/18.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | lateral VG ependymal cells - mesenchyme (B8.5 & B7.7 lines) - visceral ganglion - |
Lateral view of the head of a Ciona robusta larva co-electroporated with Dmbx>unc76::GFP (green) and Fgf8/17/18>H2B::mCherry (red) reporter constructs and COE>Vsx overexpression construct.
Nuclei from A9.30 lineage are labeled by electroporation with Fgf8/17/18>lacZ (red).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) | lateral VG ependymal cells - mesenchyme (B8.5 & B7.7 lines) - visceral ganglion - |
Lateral view of the head of a Ciona robusta larva co-electroporated with Dmbx>unc76::GFP (green) and Fgf8/17/18>H2B::mCherry (red) reporter constructs and COE>Vsx overexpression construct.
Original annotation: Vsx overexpression results in all A9.30 descendants expressing baseline levels of Dmbx>GFP (green), still visible but barely stronger than background.
| Stained molecule | KH2012:KH.C1.1212 (DMBX1; DRGX; PITX3) |
| Stained region(s) | lateral VG ependymal cells - visceral ganglion - |
Young adult probed for Fgf8/17/18 expression.
Original annotation :
Fgf8/17/18 transcript was localized to the outer ring forming the entrance to the atrial siphon (as), faintly within the atrium itself (at) and in the neural gland (ng).
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) |
Transverse section of a juvenile stage probed for Ci-FGF8/17/18 expression.
Original annotation:
Expression of Ci-FGF8/17/18 was detected in the ventral part of zone VII and ranged from the ventral boundary to the dorsal parts of zone VII.
| Stained molecule | KH2012:KH.C5.5 (FGF17; FGF18; FGF8) |
| Stained region(s) |
