Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Temporal expression profiles of 00707 at the 32-cell stage. Cluster id 00707 encodes a polypeptide with no sequence similarity.

Vegetal view of a 64-cell stage embryo probed for Ci-Pans expression.

Original annotation: the signal was present clearly in the nuclei of the A-line blastomeres.

Conversion to Aniseed format of Ghost database entry from Fujiwara S et al., Mech Dev. 2002; 112Gene name: 115-127. Original annotation: . Expression pattern: B7.7 Muscle Nervecord

Vegetal and dorsal views of a 110-cell stage embryo probed for Ci-Pans expression.

Original annotation: staining persisted in the A7.4 descendants as in the A7.8 descendants, while a new signal was present in two B-line muscle precursor pairs. Note the absence of the signal in the a-line precursors in the dorsal view.

Temporal expression profiles of 00707 at the 110-cell stage. Cluster id 00707 encodes a polypeptide with no sequence similarity. Expression in b8.17 cell pair is very weak.

Dorsal view of a gastrula stage embryo probed for Ci-Pans expression.

Dorsal-lateral view of a gastrula stage embryo probed for Ci-Pans expression.

Original annotation: expression in the muscle blastomeres disappeared and the signal remained restricted to the nervous system. Indeed, shortly after the onset of gastrulation, some a-line blastomeres, just posterior to the A-line positive row, started to be coloured: these were cells whose fate is directed to the anterior sensory vesicle, palps precursors and most probably the b8.19.

Temporal expression profiles of 00707 at the middle gastrulael stage. Cluster id 00707 encodes a polypeptide with no sequence similarity. Expression is restricted to the nerve cord lineage cells.

Vegetal view of a mid gastrula stage embryo, electroporated with CiPans-GFP construct.

Conversion to Aniseed format of Ghost database entry from Fujiwara S et al., Mech Dev. 2002; 112Gene name: 115-127. Original annotation: . Expression pattern: Nervous system

Dorsal view of a neurula stage embryo probed for Ci-Pans expression.

Original annotation: staining persisted in the a9.33-a9.34, in the a9.37-a9.38 pairs and in spots located symmetrically at the edges of the neural folds that could be homologue to the Ci-POU-IV-expressing cells.

Aniseed annotation: progenies of a9.33 are a10.65 and a10.66 cell pairs and progenies of a9.37 are a10.73 and a10.74 cell pairs. The neural folds expressing Ci-Pans could be precursors of visceral ganglion and tail nerve cord (A line neural plate).

Vegetal view of a neurula stage embryo, electroporated with CiPans-GFP construct.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: not clear

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: not clear

Dorsal and lateral views of an early tailbud probed for Ci-Pans expression.

Original annotation: as neurulation progressed, the signal remained localized in "a-line" and "A-line" neural descendants and the stain became very intense, up to delineate, at the larval stage, the antory sensory vesicle, the visceral ganglion and spots in the lateral rows of the neural tube.

Lateral view of an early tailbud stage embryo, electroporated with CiPans-GFP construct.

Lateral view of an early tailbud stage embryo stained with miR-124. Expression was observed in the central nervous system.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Neuron

Lateral view of a mid tailbud embryo probed for Ci-Pans expression.

Original annotation: as neurulation progressed, the signal remained localized in "a-line" and "A-line" neural descendants and the stain became very intense, up to delineate, at the larval stage, the antory sensory vesicle, the visceral ganglion and spots in the lateral rows of the neural tube.

Lateral view of a mid tailbud stage embryo stained with miR-124. Expression was observed in epidermal sensory neurones.

Lateral view of a late tailbud stage embryo stained with miR-124. Expression was observed in the nervous system.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Neuron

Conversion to aniseed format of DBTGR database entry from Kusakabe team.

Original annotation:
Brain vesicle.

Conversion to Aniseed format of Ghost database entry from Kusakabe T et al., Dev Biol. 2002; 242:188-203. Original annotation: . Expression pattern: Brain Ganglion

Dorsal view and high magnification of a tadpole probed for Ci-Pans expression.

Original annotation: as neurulation progressed, the signal remained localized in "a-line" and "A-line" neural descendants and the stain became very intense, up to delineate, at the larval stage, the antory sensory vesicle, the visceral ganglion and spots in the lateral rows of the neural tube.

Lateral view of a larva, electroporated with CiPans-GFP construct.

Expression of CiPans-GFP construct in electroporated Ciona embtyos at different juenile stages.