Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :AP-2-like2.
Expression pattern : No signal was detected.

WT Ciona robusta 2-cell stage embryo (anterior to the left) probed for Tfap2-r.b expression.
No Tfap2-r.b expression is observed.

WT Ciona robusta 4-cell stage embryo (anterior to the left) probed for Tfap2-r.b expression.
No Tfap2-r.b expression is observed.

WT Ciona robusta 4-cell stage embryo (anterior to the left) probed for Tfap2-r.b expression.
No Tfap2-r.b expression is observed.

WT Ciona robusta 8-cell stage embryo (anterior to the left) probed for Tfap2-r.b expression.
No Tfap2-r.b expression is observed.

WT Ciona robusta 8-cell stage embryo (anterior to the left) probed for Tfap2-r.b expression.
No Tfap2-r.b expression is observed.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :AP-2-like2.
Expression pattern : no zygotic signal has been detected.

"In normal embryos and embryos injected with the control MO, Tfap2-r.b (AP-2-like2) is expressed in the AD."

Animal view of a WT Ciona robusta 16-cell stage embryo probed for Tfap2-r.b expression (and injected with in vitro-transcribed GFP:H2B control mRNA).
Tfap2-r.b expression is observed in a5.3, a5.4, b5.3 and b5.4 blastomers.

Animal view of a WT Ciona robusta 16-cell stage embryo probed for AP2-like2 expression.
AP2-like2 is expressed in a5.3, a5.4, b5.3 and b5.4 blastomers pairs.
Original annotation: The absence of expression in A5.2 is indicated by white arrowheads. A schematic representation of the 16-cell embryo is shown on the right.

Animal view of a WT Ciona robusta 16-cell stage embryo probed for AP2-like2 expression.
AP2-like2 is expressed in a5.3, a5.4, b5.3 and b5.4 blastomers pairs and in A5.2 on the right hemisphere.
Original annotation: Expression in A5.2 is shown by a blue arrowhead and the absence of expression in A5.2 is indicated by white arrowheads. A schematic representation of the 16-cell embryo is shown on the right.

Animal view of a WT Ciona robusta 16-cell stage embryo probed for AP2-like2 expression.
AP2-like2 is expressed in a5.3, a5.4, b5.3 and b5.4 blastomers pairs.
Original annotation: The absence of expression in A5.2 is indicated by white arrowheads. A schematic representation of the 16-cell embryo is shown on the right.

Animal view of a WT Ciona robusta 16-cell stage embryo probed for AP2-like2 expression.
AP2-like2 is expressed in a5.3, a5.4, b5.3 and b5.4 blastomers pairs and in A5.2 on the right hemisphere.
Original annotation: Expression in A5.2 is shown by a blue arrowhead and the absence of expression in A5.2 is indicated by white arrowheads. A schematic representation of the 16-cell embryo is shown on the right.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :AP-2-like2.
Expression pattern : A5.2, a5.3, a5.4, b5.3, b5.4.

The illustration on the left depicts the construct. The green boxes indicate the Gfp reporter gene and SV40 polyadenylation signal. Graphs show the percentage of blastomeres expressing the reporter in the anterior and posterior vegetal blastomeres, and in the animal blastomeres.
"The 1513 bp upstream region of Tfap2-r.b (AP-2-like2) drove reporter expression specifically in the AD (S7B Fig), and a clear peak of Gata.a binding was observed within this region."

Ventral view of a late 16-cell stage embryo probed for AP2L2 expression.
Original annotation : Expression of Ci-AP2L2 was examined by WISH. The embryos were imaged from the ventral side. The anterior end is toward the top. The expression of AP2L2 in the precursors of epidermal cells was observed from the blastula stage until the end of the gastrula stage.

Animal (left) and vegetal (right) views of a WT Ciona robusta 32-cell stage embryo probed for Tfap2-r.b expression.
Tfa2-r.b is expressed in all animal blastomeres.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :AP-2-like2.
Expression pattern : a6.5, a6.6, a6.7, a6.8, b6.5, b6.6, b6.7, b6.8.

Ventral view of a late 32-cell stage embryo probed for AP2L2 expression.
Original annotation : Expression of Ci-AP2L2 was examined by WISH. The embryos were imaged from the ventral side. The anterior end is toward the top. The expression of AP2L2 in the precursors of epidermal cells was observed from the blastula stage until the end of the gastrula stage.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :AP-2-like2.
Expression pattern : a7.11, a7.12, a7.13, a7.14, a7.15, a7.16, b7.11, b7.12, b7.13, b7.14, b7.15, b7.16.

Ventral view of a 64-cell stage embryo probed for AP2L2 expression.
Original annotation : Expression of Ci-AP2L2 was examined by WISH. The embryos were imaged from the ventral side. The anterior end is toward the top. The expression of AP2L2 in the precursors of epidermal cells was observed from the blastula stage until the end of the gastrula stage.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :AP-2-like2.
Expression pattern : a8.21, a8.22, a8.23, a8.24, a8.25, a8.26, a8.27, a8.28, a8.29, a8.30, a8.31, a8.32, b8.21, b8.22, b8.24, b8.25, b8.26, b8.27, b8.28, b8.29, b8.30, b8.31, b8.32.

Ventral view of a 112-cell stage embryo probed for AP2L2 expression.
Original annotation : Expression of Ci-AP2L2 was examined by WISH. The embryos were imaged from the ventral side. The anterior end is toward the top. The expression of AP2L2 in the precursors of epidermal cells was observed from the blastula stage until the end of the gastrula stage.

Animal and lateral view with anterior to the top of a 10-stage embryo stained with Ap2-like2 (KH.C7.43) in DMSO. In the panel, vegetal cells are marked in grey, anterior ectoderm in white, posterior ectoderm in yellow and gene expression in blue. Expression was observed in the whole embryo except for the a8.17, a8.19, a8.25, b8.17, b8.18, b8.19 and b8.20 cell pairs.

Views of early gastrula stage embryos probed for reporter expression of the region upstream of Ci-AP-2-like2.

No expression was detected.

Ventral view of an early gastrula probed for AP2L2 expression.
Original annotation : Expression of Ci-AP2L2 was examined by WISH. The embryos were imaged from the ventral side. The anterior end is toward the top. The expression of AP2L2 in the precursors of epidermal cells was observed from the blastula stage until the end of the gastrula stage.

Animal view and lateral view of an early gastrula stage embryo stained with Ap2-like2.

Ventral view of a mid gastrula embryo probed for AP2L2 expression (pseudocolored in magenta). Nuclei are shown by DAPI (green)
Original annotation : Expression of AP2L2 was examined at 5.5 hpf by FISH. The embryos were imaged from the ventral side with the anterior end toward the right.
The graph shows quantified expression level : The expression of AP2L2 along the A-P axis was quantified from the image. Normalized fluorescence intensities averaged along the D-V axis are shown for different embryos as line plots of different colors. The black-line plots represent the intensity quantified in the FISH image (a.u., arbitrary units).

Experimental conditions description : Animal view of a mid gastrula embryo probed for AP2L2 expression (pseudocolored in magenta). Nuclei are shown by DAPI (green)
Original annotation : Original annotation : Expression of GATAb was examined at 5.5 hpf by FISH. The expression of Ci-AP2L2 in all the precursors of epidermal cells was observed from the blastula stage until the end of the gastrula stage. The embryos were imaged from the ventral side with the anterior end toward the right.
The graph shows quantified expression level : The expression of GATAb along the A-P axis was quantified from the image. Normalized fluorescence intensities averaged along the D-V axis are shown for different embryos as line plots of different colors. The black-line plots represent the intensity quantified in the FISH image (a.u., arbitrary units).
The fluorescence intensity in the picture is represented as a heatmap (3rd image).

Conversion to Aniseed format of data from Yutaka Satous gene expression profiles of transcription factors and signalling molecule ISH screen(Development 131(16).

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :AP-2-like2.
Expression pattern : epidermis.

Ventral view of a late gastrula probed for AP2L2 expression.
Original annotation : Expression of Ci-AP2L2 was examined by WISH. The embryos were imaged from the ventral side. The anterior end is toward the top. The expression of AP2L2 in the precursors of epidermal cells was observed from the blastula stage until the end of the gastrula stage.

Ventral view of an early neurula probed for AP2L2 expression.
Original annotation : Expression of Ci-AP2L2 was examined by WISH. The neurula embryos were imaged from the left lateral side. The anterior end is toward the top. The expression of AP2L2 in the precursors of epidermal cells was observed from the blastula stage until the end of the gastrula stage.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :AP-2-like2.
Expression pattern : epidermis.

No picture avaible. Ap2-like2 is expressed in the A7.6/TLC lineage at the neurula stage.

Ventral view of a late neurula probed for AP2L2 expression.
Original annotation : Expression of Ci-AP2L2 was examined by WISH. The neurula embryos were imaged from the left lateral side. The anterior end is toward the top. The expression of AP2L2 in the precursors of epidermal cells was observed from the blastula stage until the end of the gastrula stage.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Mesenchyme

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :AP-2-like2.
Expression pattern : epidermis.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: not clear

Views of early tailbud stage embryos showing reporter expression of the region upstream of AP2-like in tail epidermis.

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :AP-2-like2.
Expression pattern : epidermis.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Endoderm

Views of larvae showing reporter expression of the region upstream of AP2-like in tail epidermis.