Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tbx1.
Expression pattern : Unlocalized signal was detected.
Comment : Ubiquitous staining was observed throughout embryogenesis. This expression is likely maternal.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tbx1.
Expression pattern : No distinct zygotic signal was detected.
Comment : Ubiquitous staining was observed throughout embryogenesis. This expression is likely maternal.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tbx1.
Expression pattern : No distinct zygotic signal was detected.
Comment : Ubiquitous staining was observed throughout embryogenesis. This expression is likely maternal.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tbx1.
Expression pattern : No distinct zygotic signal was detected.
Comment : Ubiquitous staining was observed throughout embryogenesis. This expression is likely maternal.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tbx1.
Expression pattern : No distinct zygotic signal was detected.
Comment : Ubiquitous staining was observed throughout embryogenesis. This expression is likely maternal.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tbx1.
Expression pattern : No distinct zygotic signal was detected.
Comment : Ubiquitous staining was observed throughout embryogenesis. This expression is likely maternal.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tbx1.
Expression pattern : No distinct zygotic signal was detected.
Comment : Ubiquitous staining was observed throughout embryogenesis. This expression is likely maternal.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tbx1.
Expression pattern : No distinct zygotic signal was detected.
Comment : Ubiquitous staining was observed throughout embryogenesis. This expression is likely maternal.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tbx1.
Expression pattern : nervous system..
Comment : Ubiquitous staining was observed throughout embryogenesis. This expression is likely maternal.

Expression of Ci-Tbx1 was detected by whole-mount in situ hybridization. View of an early tailbud stage embryo.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tbx1.
Expression pattern : nervous system.
Comment : Ubiquitous staining was observed throughout embryogenesis. This expression is likely maternal.

Expression of Ci-Tbx1 was detected by whole-mount in situ hybridization. View of a mid tailbud stage embryo.

Expression of Ci-Tbx1 was detected by whole-mount in situ hybridization. View of a mid tailbud stage embryo.

Expression of Ci-Tbx1 was detected by whole-mount in situ hybridization. View of a late tailbud stage embryo. In the late tailbud stage embryo, the signal was evident in aprroximately 10 dorsal endodermal cells.

Magnified view of longitudinal muscles (LoMs, arrowhead) segregating from atrial siphon muscles (ASMs, arrows) during metamorphosis, visualized by MesP>lacZ expression (red) and in situ hybridization of Tbx1/10 (green). Panel width is ~100 μm.
(D) Mesp>LacZ expression
(E) In situ hybridization of Tbx1/10 (green).
(F) Merged view of (D) and (E), showing activation of Tbx1/10 in LoMs.

In situ hybridization of Tbx1/10 (green) in two Ciona robusta juveniles expressing MesP>lacZ (red) to visualize the differentiated B7.5 lineage.
Tbx1/10 is expressed in longitudinal muscles (lom) precursors segregating from the atrial siphon muscles (asm). (as: atrial siphon, Ht: Heart, asterisk: reabsorbed tail).
BD/B'D': Close up of boxed area in A/A'
B/B': MesP>lacZ
C/C' Tbx1/10 in situ
D/D' merged image.