Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Orphan Wnt e.
Expression pattern : No signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Orphan Wnt e.
Expression pattern : B5.1

Illustrations on the left depict the constructs. Green boxes indicate the Gfp reporter gene and SV40 polyadenylation signal. The numbers indicate the relative nucleotide positions from the transcription start site of Wnttun5. Mutant Tcf7-binding sites are indicated by X. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, in the posterior vegetal blastomeres, and in the animal blastomeres.

Illustrations on the left depict the constructs. Green boxes indicate the Gfp reporter gene and SV40 polyadenylation signal. The numbers indicate the relative nucleotide positions from the transcription start site of Wnttun5. Mutant Tcf7-binding sites are indicated by X. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, in the posterior vegetal blastomeres, and in the animal blastomeres.

Illustrations on the left depict the constructs. Green boxes indicate the Gfp reporter gene and SV40 polyadenylation signal. The numbers indicate the relative nucleotide positions from the transcription start site of Wnttun5. Mutant Tcf7-binding sites are indicated by X. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, in the posterior vegetal blastomeres, and in the animal blastomeres.
Other imgae : Images showing expression of the reporter in embryos electroporated with (C) the third and (D) last constructs shown in (B). Scale bar, 100 μm.
"A 188 bp region within the upstream sequence of Wnttun5 (−792 to −979) was necessary for specific expression in the PVD"

Illustrations on the left depict the constructs. Green boxes indicate the Gfp reporter gene and SV40 polyadenylation signal. The numbers indicate the relative nucleotide positions from the transcription start site of Wnttun5. Mutant Tcf7-binding sites are indicated by X. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, in the posterior vegetal blastomeres, and in the animal blastomeres.

Illustrations on the left depict the constructs. Green boxes indicate the Gfp reporter gene and SV40 polyadenylation signal. The numbers indicate the relative nucleotide positions from the transcription start site of Wnttun5. Mutant Tcf7-binding sites are indicated by X. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, in the posterior vegetal blastomeres, and in the animal blastomeres.
Mutations introduced into either of two putative Tcf7-binding sites greatly reduced expression

Illustrations on the left depict the constructs. Green boxes indicate the Gfp reporter gene and SV40 polyadenylation signal. The numbers indicate the relative nucleotide positions from the transcription start site of Wnttun5. Mutant Tcf7-binding sites are indicated by X. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, in the posterior vegetal blastomeres, and in the animal blastomeres.
Mutations introduced into either of two putative Tcf7-binding sites greatly reduced expression
Other image : Images showing expression of the reporter in embryos electroporated with (C) the third and (D) last constructs shown in (B). Scale bar, 100 μm.

Illustrations on the left depict the constructs. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, and in the posterior vegetal blastomeres.
“While a construct containing a 297 bp upstream sequence between −994 and −697 drove reporter expression specifically in the PVD, constructs containing shorter upstream sequences (−994 to −735, −994 to −793, −994 to −853, and −979 to −860) drove reporter expression in both the AVD and PVD”

Illustrations on the left depict the constructs. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, and in the posterior vegetal blastomeres.
“While a construct containing a 297 bp upstream sequence between −994 and −697 drove reporter expression specifically in the PVD, constructs containing shorter upstream sequences (−994 to −735, −994 to −793, −994 to −853, and −979 to −860) drove reporter expression in both the AVD and PVD”

Illustrations on the left depict the constructs. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, and in the posterior vegetal blastomeres.
“While a construct containing a 297 bp upstream sequence between −994 and −697 drove reporter expression specifically in the PVD, constructs containing shorter upstream sequences (−994 to −735, −994 to −793, −994 to −853, and −979 to −860) drove reporter expression in both the AVD and PVD”

Illustrations on the left depict the constructs. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, and in the posterior vegetal blastomeres.
“While a construct containing a 297 bp upstream sequence between −994 and −697 drove reporter expression specifically in the PVD, constructs containing shorter upstream sequences (−994 to −735, −994 to −793, −994 to −853, and −979 to −860) drove reporter expression in both the AVD and PVD”

Illustrations on the left depict the constructs. Graphs show the percentage of blastomeres expressing the reporter in the anterior vegetal blastomeres, and in the posterior vegetal blastomeres.
“While a construct containing a 297 bp upstream sequence between −994 and −697 drove reporter expression specifically in the PVD, constructs containing shorter upstream sequences (−994 to −735, −994 to −793, −994 to −853, and −979 to −860) drove reporter expression in both the AVD and PVD”

First picture
The repressive element was inserted into −1041 of the upstream sequence of Foxd.b. The graphs indicate that this insertion made the expression of the reporter specific for the posterior vegetal cells. Because no expression in the animal hemisphere was observed with the constructs shown in (C), (D) and (E), graphs for expression in the animal hemisphere are omitted.
Second picture
Image showing expression of the reporter with the second construct shown in (E). Scale bar, 100 μm.
"Thus, similar to Tbx6.b, Wnttun5 also has a cis-regulatory element that repressed the reporter expression in the AVD."

Vegetal view of a late 32-cell stage embryo. Orphan Wnt-e expression was detected in primary muscle-lineage cells.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Orphan Wnt e.
Expression pattern : B6.2

Aniseed annotation :
According to the picture, it seems that there is also expression in B6.4 cell pair.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Orphan Wnt e.
Expression pattern : B7.4, B7.8

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Orphan Wnt e.
Expression pattern : B8.7, B8.8, B7.5, B8.15, B8.16

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Orphan Wnt e.
Expression pattern : muscle

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Orphan Wnt e.
Expression pattern : muscle, nervous system

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Orphan Wnt e.
Expression pattern : nervous system, nerve cord.
Comment : the most anterior neural cells in the brain and the most posterior nerve cord cells.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Orphan Wnt e.
Expression pattern : unidentifiable.
Comment : cells around the tip of the tail