Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxC.
Expression pattern : No signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxC.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxC.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxC.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxC.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxC.
Expression pattern : a8.17, a8.19

Aniseed annotation :
According to the other developmental stages and to the picture, it seems that expression is in a8.18 and a8.20 cell pairs.

Views of early gastrula stage embryos showing reporter expression of the FoxC promoter in a8.20 cells.

View of a mid-gastrula stage embryo stained with FoxC driving GFP (green) and hoechst for DNA (blue). FoxC expression is observed in rows V and VI.

Vegetal view of a mid gastrula stage embryo probed for FoxC expression.

Vegetal view of a mid gastrula stage wild type embryo probed for FoxC expression.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxC.
Expression pattern : nervous system.
Comment : a9.35, a9.36, a9.39, a9.40

Vegetal view of a late gastrula stage embryo showing Ci-FoxC expression.

At the late gastrula stage, FoxC is expressed in cells from rows V and VI of the neural plate
Original annotation : The expression of FoxC in control embryos at the late gastrula stage (shown in animal view). The expression is illustrated schematically in the lower right by red (palps) and blue (brain).

Views of late gastrula stage embryos showing reporter expression of the region upstream of Ci-FoxC (from -493bo to -71bp) in a9.35, a9.36, a9.39 and a9.40 cells.

Views of late gastrula stage embryos showing reporter expression of the region upstream of Ci-FoxC in a9.35, a9.36, a9.39 and a9.40 cells.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxC.
Expression pattern : nervous system

Aniseed annotation :
It seems that the staining is localised in the precurors of the palps and in the precursors of the lateral anterior sensory vesicles.

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxC.
Expression pattern : nervous system.
Comment : anterior part of the brain

Aniseed annotation :
Very weak expression is detected in the palps.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxC.
Expression pattern : nervous system.
Comment : anterior part of the brain, palp

Views (dorsal view for the second picture) of mid tailbud stage embryos showing reporter expression of the region upstream of FoxC in anterior sensory vesicle and in palps.

Aniseed Annotation : According to the first picture it seems that the majority of embryos didn't show anterior sensory vesicle's staining.

Mid tailbud embryo where Islet>mCherry reporter (Red) was co-expressed with FoxC>YFP-caax (Green)and FoxC>lacZ. YFP-caax is a membrane-targeted reporter gene, here expressed in the palps.

Mid tailbud embryo probed for Emx expression (red) in the presence of FoxC>LacZ control and FoxC>GFPcaax reporter (green). Nuclei stained with Hoechst. The reporter construct FoxC>GFP-caax drives GFP expression in the palps, targetting the plasma membranes.

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Views of late tailbud stage embryos showing reporter expression of the region upstream of Ci-FoxC (from -1023bp to -71bp) in anterior sensory vesicle and in palps.

Aniseed Annotation : According to the picture just few embryos showed a very weak signal in the sensory vesicle.

View of a tailbud stage embryo stained with FoxC driving GFP (green). FoxC expression is observed in the palps.
Original annotation : FoxC expression is maintained in the progeny of a8.18 and a8.20 blastomeres, namely a9.35, a9.36, a9.39 and a9.40, which comprise the medial four columns of the anteriormost neural plate, rows V and VI. We have isolated a ∼2 kb FoxC enhancer upstream of the FoxC coding sequence and found it to recapitulate the native expression pattern.

Dorsal view of a late-tailbud stage embryo stained with FoxC driving GFP (green). The approximate outline of the brain (blue), and embryo is indicated by the dashed lines (orange). Expression was observed in the palps.

Views of larvae showing reporter expression of the region upstream of FoxC in anterior sensory vesicle and in palps.

Views of larvae showing reporter expression of the region upstream of FoxC in anterior sensory vesicle and in palps.

Hatching larva electroporated with FoxC>H2B:Cherry and Islet>YFPcaax reporter plasmids and stained with phalloidin in control conditions (ie co-electroporated with FoxC>LacZ
Original annotation : Expression of the control transgene FoxC>lacZ does not alter palp morphogenesis, as visualized by FoxC>H2B:Cherry (nucleus-targetted mCherry).

Original annotation : Larva electroporated with DMRT>Cherry-caax (Red) and FoxC>H2B:CFP (Blue, targetting nuclei) to label anterior neural plate and palp lineage, respectively. Control larva expressing DMRT>lacZ and FoxC>lacZ shows normal palp development.

Original annotation :Larva expressing FoxC>H2B:mCherry (red) and palp differentiation marker βγ-crystallin>GFP (green). Control larva (co-electroporated with DMRT>LAcZ and FoxC>LacZ) shows βγ-crystallin reporter in two of the three palps but not in the intervening palp domain marked by FoxC reporter.