Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Achaete-Scute a-like2.
Expression pattern : No signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Achaete-Scute a-like2.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Achaete-Scute a-like2.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Achaete-Scute a-like2.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Achaete-Scute a-like2.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Achaete-Scute a-like2.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Achaete-Scute a-like2.
Expression pattern : nervous system

Aniseed annotation :
Expression at the tip of the tail: caudal epidermal neurons or posterior most tail epidermis?

Dorsal and ventral view.

Original annotation: no expression of Ascl.b. Ventral and lateral views are shown.

Original annotation: expression in the ventral tail ectoderm. Ventral and lateral views are shown.
Aniseed annotation: expression in the ventral and dorsal tail ectoderm and palps.

“Achaete-scute a-like 2 was expressed in the palp forming region.”

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Dorsal view of a tailbud stage wild type embryo probed for Ash2 expression.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Achaete-Scute a-like2.
Expression pattern : nervous system, epidermis.
Comment : epidermis along the dosal and ventral midline of the tail, and plap

Original annotation: lacZ MO injection doesn't affect Ascl.b expression. expression in the tail ventral ectoderm and in the dorsal ectoderm. Lateral view is shown.
Aniseed annotation: expression in the tail ventral ectoderm, in the dorsal ectoderm, and at the anterior (palps)

Original annotation: expression throughout the tail ventral ectoderm.

“Achaete-scute a-like 2 was expressed in the palp forming region.”

Views of early tailbud stage embryos showing reporter expression of the region upstream of Achaete-scute in mesenchyme and weak staining in anterior sensory vesicle and part of muscle only in few embryos.

Lateral view of an early tailbud stage embryo stained with Ash.
Original annotation: Expression was observed in the dorsal and ventral midline epidermis.

“Achaete-scute a-like 2 was expressed in the palp forming region.”

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Achaete-Scute a-like2.
Expression pattern : nervous system.
Comment : epidermis along the dosal and ventral midline of the tail, and palps.

“Achaete-scute a-like 2 was expressed in the palp forming region.”

Lateral view of a mid tailbud stage embryo stained with Ash.
Original annotation: No expression was observed in the embryo.
Aniseed annotation: Expression was observed in the palps.

“Achaete-scute a-like 2 was expressed in the palp forming region.”

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Lateral view of a mid tailbud stage embryo stained with Ash. White lines demarcate boundaries between different focal planes.
Original annotation: No expression was observed in the embryo.
Aniseed annotation: Expression was observed in the palps.

Views of larvae showing reporter expression of the region upstream of Achaete-scute in mesenchyme, anterior sensory vesicle and part of muscle.

Aniseed Annotation : According to the pictures it seems that there is head endoderm staining in some embryos.