1 result
OVERVIEW
Click here to see the RNA-Seq Pipeline Protocol.
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Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Halocynthia roretzi (Aomori, Japan) were fertilized by sperms of an another single individual and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
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| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 15 (mid neurula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
10 results
Immunostaining of macho-1 protein in a WT Halocynthia roretzi 4-cell stage embryo, anterior to the left.
A signal was observed in the B-line at the CAB (cellular cortical structure known as centrosome-attracting body)
Scale bar: 100 μm.
| Stained molecule | Harore.CG.MTP2014.S236.g13376 (ZIC2; ZIC3; ZIC4) |
| Stained region(s) | B3 cell pair - |
Immunostaining of macho-1 protein in a WT Halocynthia roretzi 8-cell stage embryo, lateral view with anterior to the left.
A signal was observed in the B-line at the CAB (cellular cortical structure known as centrosome-attracting body)
| Stained molecule | Harore.CG.MTP2014.S236.g13376 (ZIC2; ZIC3; ZIC4) |
| Stained region(s) | B4.1 cell pair - |
Immunostaining of macho-1 protein in a Halocynthia roretzi 8-cell stage embryo injected with a control MO, lateral view with anterior to the left.
A signal was observed in the B-line at the CAB (cellular cortical structure known as centrosome-attracting body, arrowhead).
Scale bar: 100 μm.
| Stained molecule | Harore.CG.MTP2014.S236.g13376 (ZIC2; ZIC3; ZIC4) |
| Stained region(s) | B4.1 cell pair - |
Immunostaining of macho-1 protein in a WT Halocynthia roretzi 8-cell stage embryo, lateral view with anterior to the left.
A signal was observed in the B-line at the CAB (cellular cortical structure known as centrosome-attracting body)
| Stained molecule | Harore.CG.MTP2014.S236.g13376 (ZIC2; ZIC3; ZIC4) |
| Stained region(s) | B4.1 cell pair - |
Immunostaining of macho-1 protein in a Halocynthia roretzi 8-cell stage embryo injected with a control MO, lateral view with anterior to the left.
A signal was observed in the B-line at the CAB (cellular cortical structure known as centrosome-attracting body, arrowhead).
Scale bar: 100 μm.
| Stained molecule | Harore.CG.MTP2014.S236.g13376 (ZIC2; ZIC3; ZIC4) |
| Stained region(s) | B4.1 cell pair - |
Immunostaining of macho-1 protein in a Halocynthia roretzi 8-cell stage embryo injected with macho-1 MO, lateral view with anterior to the left
WT macho-1 signal at the CAB is specific to macho-1, since it was no longer observed in embryos where macho-1 had been knocked down by MO injection.
| Stained molecule | Harore.CG.MTP2014.S236.g13376 (ZIC2; ZIC3; ZIC4) |
| Stained region(s) | No expression - |
No embryo picture in the article.
Macho-1 is expressed in the B4.1 blastomeres, in the CAB (cellular cortical structure known as centrosome-attracting body).
| Stained molecule | Harore.CG.MTP2014.S236.g13376 (ZIC2; ZIC3; ZIC4) |
| Stained region(s) | B4.1 cell pair - |
Posterior view with animal pole up of a Halocynthia roretzi 8-cell stage embryo probed for PEM expression and injected with Hr-β-catenin MO.
Macho-1 is expressed in the B4.1 blastomere.
Original comment: Reduction in size of the area of RNA localization was also evident in single-knockdown embryos that had been treated with β-catenin MO.
| Stained molecule | Harore.CG.MTP2014.S236.g13376 (ZIC2; ZIC3; ZIC4) |
| Stained region(s) | B4.1 cell pair - |
Immunostaining of macho-1 protein in a WT Halocynthia roretzi 16-cell stage embryo, vegetal view with anterior up.
A signal is observed in the B5.2 blastomeres at the CAB (cellular cortical structure known as centrosome-attracting body).
| Stained molecule | Harore.CG.MTP2014.S236.g13376 (ZIC2; ZIC3; ZIC4) |
| Stained region(s) | B5.2 cell pair - |
Immunostaining of macho-1 protein in a WT Halocynthia roretzi 32-cell stage embryo, vegetal view with anterior up.
A signal is observed in the B6.3 blastomeres at the CAB (cellular cortical structure known as centrosome-attracting body).
| Stained molecule | Harore.CG.MTP2014.S236.g13376 (ZIC2; ZIC3; ZIC4) |
| Stained region(s) | B6.3 cell pair - |
Immunostaining of macho-1 protein in a WT Halocynthia roretzi 64-cell stage embryo, vegetal view with anterior up.
A signal is observed in the B7.6 blastomeres at the CAB (cellular cortical structure known as centrosome-attracting body).
| Stained molecule | Harore.CG.MTP2014.S236.g13376 (ZIC2; ZIC3; ZIC4) |
| Stained region(s) | B7.6 cell pair - |
Immunostaining of macho-1 protein in a WT Halocynthia roretzi 110-cell stage embryo, vegetal view with anterior up.
A signal is observed in the B7.6 blastomeres at the CAB (cellular cortical structure known as centrosome-attracting body).
| Stained molecule | Harore.CG.MTP2014.S236.g13376 (ZIC2; ZIC3; ZIC4) |
| Stained region(s) | B7.6 cell pair - |
