Lateral view of a WT Molgula occidentalis mid tailbud embryo (dotted outline) probed for Vsx (green) and Mnx (pink) expressions. Vsx is expressed in A11.119 and A11.117 cell pairs.
Orignal annotation : Two-color fluorescent in situ hybridization reveals alternating expression of interneuron marker Vsx (green) in undifferentiated A11.119 interneuron progenitor cell and interneuon 2 (IN2) and motor neuron marker Mnx (magenta) in motor neurons 1 and 2 (MN1, MN2). Only one side of embryo is shown. Inset is a magnified view of dashed box.Scale bar = 25 µm

Lateral view of a WT Molgula occidentalis mid tailbud embryo (dotted outline) probed for Mnx (pink) and Vsx (green) expressions. Mnx is expressed in A11.118 and A10.64 cell pairs.
Orignal annotation : Two-color fluorescent in situ hybridization reveals alternating expression of interneuron marker Vsx (green) in undifferentiated A11.119 interneuron progenitor cell and interneuon 2 (IN2) and motor neuron marker Mnx (magenta) in motor neurons 1 and 2 (MN1, MN2). Only one side of embryo is shown. Inset is a magnified view of dashed box.Scale bar = 25 µm

Lateral view of a WT Molgula occidentalis mid tailbud embryo probed for Vsx (green) and Islet (pink) expressions. Vsx is expressed in A11.119 and A11.117 cell pairs.
Orignal annotation : Interneuron marker Vsx (green) is expressed in undifferentiated A11.119 interneuron progenitor cell and interneuron 2 (IN2).
Boxed area magnified in insert. Anterior is to the left. Scale bar = 25 µm

Lateral view of a WT Molgula occidentalis mid tailbud embryo probed for Islet (pink) and Vsx (green) expressions. Islet is expressed in A10.64 cell pair (or motor neuron 2 MN2) and in the notochord.
Boxed area magnified in insert. Anterior is to the left. Scale bar = 25 µm

Lateral view of a WT Molgula occidentalis mid tailbud embryo probed for Lhx3/4 (green) and Vsx (magenta) expressions. Lhx3/4 is expressed in the visceral ganglion, in the motor neuron 2 (MN2) and in the anterior sensory vesicle.
Orignal annotation : Two-color fluorescent in situ hybridization for Lhx3/4.a (green) and Vsx (magenta) showing strong co-expression in IN2, and diffuse Lhx3/4.a expression throughout the motor ganglion.
Anterior is to the left. Boxed area is enlarged in inserts. Scale bar = 25 µm

Lateral view of a WT Molgula occidentalis mid tailbud embryo probed for Lhx3/4 (green) and Vsx (magenta) expressions. Vsx is expressed in A11.119 and A11.117 cell pairs (respectively interneuron 1 precursor and interneuron 2 (IN2)).
Orignal annotation : Two-color fluorescent in situ hybridization for Lhx3/4.a (green) and Vsx (magenta) showing strong co-expression in IN2, and diffuse Lhx3/4.a expression throughout the motor ganglion.
Anterior is to the left. Boxed area is enlarged in inserts. Scale bar = 25 µm

Lateral view of the head/neck a WT Molgula occidentalis mid tailbud embryo probed for Vsx (green) and Pax3/7 (magenta) expressions. Vsx is expressed in A11.119 and A11.117 cell pairs (respectively interneuron 1 precursor and interneuron 2).
Anterior is to the left. Boxed area is enlarged in insert.

Lateral view of the head of a WT Molgula occidentalis mid tailbud embryo probed for Vsx (green) and Pax3/7 (magenta) expressions. Pax3/7 is expressed in the A11.120 cell pair (anterior part of the visceral ganglion) and in the anterior sensory vesicle.
Orignal annotation : The expression of Pax3/7 is anterior and adjacent to Vsx expression in the A11.119 cell. Pax3/7 is thus deduced to be expressed in the sister cell of A11.119, A11.120. This is identical to its expression pattern in Ciona.
Anterior is to the left. Boxed area is enlarged in insert.

Dorsal view of the head of a WT Molgula occidentalis mid tailbud embryo probed for Vsx (green) and Dmbx (magenta) expressions. Vsx is expressed in A11.119 and A11.117 cell pairs (respectively interneuron 1 precursor and interneuron 2).
Anterior is to the left. Boxed area is enlarged in insert.

Lateral view of a WT Molgula occidentalis mid tailbud embryo probed for Vsx (green) and Dmbx (magenta) expressions. Dmbx is expressed in A11.120 cell pair (anterior part of the visceral ganglion).
Original annotation : This double in situ shows expression of Dmbx in A11.120 (like Pax3/7). This is different from the situation in Ciona, in which Dmbx is detected only later, in the descending decussating neuron (ddN, equivalent to A12.239, the posterior daughter cell of A11.120).
Anterior is to the left. Boxed area is enlarged in insert.