Lateral view of a WT Molgula occidentalis mid tailbud embryo probed for Lhx3/4 (green) and Islet (magenta) expressions. Lhx3/4 is expressed in the visceral ganglion, in the motor neuron 2 (MN2) and in the anterior sensory vesicle.
Orignal annotation : Two-color fluorescent in situ hybridization for Lhx3/4.a (green) and Islet (magenta) showing co-expression in MN2.
Anterior is to the left. Boxed area is enlarged in inserts. Scale bar = 25 µm

Lateral view of a WT Molgula occidentalis mid tailbud embryo probed for Lhx3/4 (green) and Islet (magenta) expressions. Islet is expressed in the motor neuron 2 (MN2) and in the notochord.
Orignal annotation : Two-color fluorescent in situ hybridization for Lhx3/4.a (green) and Islet (magenta) showing co-expression in MN2.
Anterior is to the left. Boxed area is enlarged in inserts. Scale bar = 25 µm

Lateral view of a WT Molgula occidentalis mid tailbud embryo probed for Lhx3/4 (green) and Vsx (magenta) expressions. Lhx3/4 is expressed in the visceral ganglion, in the motor neuron 2 (MN2) and in the anterior sensory vesicle.
Orignal annotation : Two-color fluorescent in situ hybridization for Lhx3/4.a (green) and Vsx (magenta) showing strong co-expression in IN2, and diffuse Lhx3/4.a expression throughout the motor ganglion.
Anterior is to the left. Boxed area is enlarged in inserts. Scale bar = 25 µm

Lateral view of a WT Molgula occidentalis mid tailbud embryo probed for Lhx3/4 (green) and Vsx (magenta) expressions. Vsx is expressed in A11.119 and A11.117 cell pairs (respectively interneuron 1 precursor and interneuron 2 (IN2)).
Orignal annotation : Two-color fluorescent in situ hybridization for Lhx3/4.a (green) and Vsx (magenta) showing strong co-expression in IN2, and diffuse Lhx3/4.a expression throughout the motor ganglion.
Anterior is to the left. Boxed area is enlarged in inserts. Scale bar = 25 µm