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  1. Molecular Tool 'DMRT1>BZ2'

Molecular tool card for 'DMRT1>BZ2'

Name

DMRT1>BZ2

Targeted Gene

(PRDM1; ZNF235; ZNF683)

Family

Sequence-based

Type

overexpression_construct

Supplier

n/a

Comment

This constuct is used to express BZ2 under the DMRT1 upstream sequence, leading to overexpression in the a neural plate. The cis-regulatory sequence of DMRT1 used in this construct is shown below.

Regulation

Gain of function

Publication

Ikeda et al. (2013). A time delay gene circuit is required for palp formation in the ascidian embryo. Development 140(23):4703-8

Sequence

>Ciinte.REG.KH2012.S544:4212-5239|DMRT1cttcgccggagagttaaaatc
atcattatagtagggtggaggaagatgggacacctttggcacataatatccaaatatcct
taccccgttttacacaattaacagcggtatatgggagtcgtaagaattccgttttataat
tatttgcatgttctttgtttgctactaaattggacgacaaaatgtagtcaaaaggtgtct
aatcttcccctaccctactatacgacttggggcatagttataaagttattccgtttcatt
tcttggcgttaacccgacgatcagtaattaacgatattcttcataaataaataatgcgag
aactgcaacaggactagggaagttacttaagcgagcatgtgattagaaattgccactgaa
gcactttaaaatgccgccaaaacacatacaccttcatgacacattgttttacagaatctc
aatttaaaacccgtcgacaattatcggatttccgccgcagagtcacaggaagttgttttg
cttaataatacttgttttagataagctgttatgttgctcaatgaataacaataatgttta
cgttggttgttgtggcaatgtacattgtaacttccacaaacctctcagagggcaaacaca
ttaagcaaactgaatttgccaagtacttcgggaagtcactgataagcggacaatacgaag
cgatcacagggaattgtcgcacgccagaatgtaaacaaaaacatcgcctgtgcaccgcgc
gatcgaagacacacagcgctgactaattgaccaacacaaaagcgaggaaagaaaaaaaag
cgaaaacgagaacgggatattaattgtacacgattgtgacgtaaattcgaggcatttttt
cttccgaaatcgagcggtttaaatacctcgcgtctttattaggtacaatcatattgcctt
gataccttgcttagaacagtgaatacatataaatcgtattaaaagctttagagtgttaaa
ccaaagagttacagaaattatagcaaacagttcgtttaactggcatg

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