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- Molecular Tool 'Dmrt>TGF-βR(CA)'
Molecular tool card for 'Dmrt>TGF-βR(CA)'
Name
Dmrt>TGF-βR(CA)
Targeted Gene
Family
Sequence-based
Type
overexpression_construct
Supplier
n/a
Comment
This construct is used to misexpress a constitutively active form of the transforming growth factor-β receptor (TGF-βRCA) throughout the anterior brain and adjacent ectoderm, thanks to Dmrt regulatory sequence (sequence below). The TGF-βRCA construct was made by site-directed mutagenesis to introduce a Q154D missense mutation in the regulatory glycine- and serine-rich domain of the wild type receptor.
Regulation
Gain of function
>KhS544:4240-5239_Dmrttagtagggtggaggaagatgggacacctttggcacata
atatccaaatatccttaccccgttttacacaattaacagcggtatatgggagtcgtaaga
attccgttttataattatttgcatgttctttgtttgctactaaattggacgacaaaatgt
agtcaaaaggtgtctaatcttcccctaccctactatacgacttggggcatagttataaag
ttattccgtttcatttcttggcgttaacccgacgatcagtaattaacgatattcttcata
aataaataatgcgagaactgcaacaggactagggaagttacttaagcgagcatgtgatta
gaaattgccactgaagcactttaaaatgccgccaaaacacatacaccttcatgacacatt
gttttacagaatctcaatttaaaacccgtcgacaattatcggatttccgccgcagagtca
caggaagttgttttgcttaataatacttgttttagataagctgttatgttgctcaatgaa
taacaataatgtttacgttggttgttgtggcaatgtacattgtaacttccacaaacctct
cagagggcaaacacattaagcaaactgaatttgccaagtacttcgggaagtcactgataa
gcggacaatacgaagcgatcacagggaattgtcgcacgccagaatgtaaacaaaaacatc
gcctgtgcaccgcgcgatcgaagacacacagcgctgactaattgaccaacacaaaagcga
ggaaagaaaaaaaagcgaaaacgagaacgggatattaattgtacacgattgtgacgtaaa
ttcgaggcattttttcttccgaaatcgagcggtttaaatacctcgcgtctttattaggta
caatcatattgccttgataccttgcttagaacagtgaatacatataaatcgtattaaaag
ctttagagtgttaaaccaaagagttacagaaattatagcaaacagttcgtttaactggca
tg