Name

Fgf8>RG(R818E)

Targeted Gene

(DAB2IP; RASA1; RASAL2)

Family

Sequence-based

Type

overexpression_construct

Supplier

n/a

Comment

RG(R818E) was generated by PCR-based introduction of a point mutation resulting in an arginine-to-glutamate substitution at amino acid residue 818 (Miao et al., 1996). The RG(R818E) ORF was placed under the fgf8 promoter for electroporation (Imai et al., 2009)

Regulation

Loss of function

Publication

Haupaix et al. (2013). p120RasGAP mediates ephrin/Eph-dependent attenuation of FGF/ERK signals during cell fate specification in ascidian embryos. Development 140(21):4347-52