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  1. Molecular Tool 'pBra>Onecut/HNF6'

Molecular tool card for 'pBra>Onecut/HNF6'

Name

pBra>Onecut/HNF6

Targeted Gene

(ONECUT1; ONECUT2; ONECUT3)

Family

Sequence-based

Type

overexpression_construct

Supplier

n/a

Comment

THis construct overexpresses Onecut (HNF6) in most of notochord cells. It consists of a BlueScript vector containing 700 bp of Ciona promoter region of Brachyury gene (Ci-Bra), GFP as reporter gene and SV40 polyadenylation signal. The GFP reporter gene was replaced with the full-length Ci-Onecut cDNA using EcoRI to make the Bra-Onecut construct.

Regulation

Gain of function

Publication

Pezzotti et al. (2014). Auto and cross regulatory elements control Onecut expression in the ascidian nervous system. Dev. Biol. 390(2):273-87

Sequence

No sequence

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