Name

Mesp>FGFR-DN (Davidson 2006)

Targeted Gene

(FGF16; FGF20; FGF9)

Family

Sequence-based

Type

overexpression_construct

Supplier

Comment

Mesp (KH.C3.100) full enhancer drives the dominant-negative form of Ci-FGFR (C2.125) in the B7.5 lineage. The dominant-negative form of Ci-FGFR (R1CiGC32j14)lacking the intracellular domain was generated using the following primer pair: 5'-aatggcggccgcaATGATACAACTACAAAATACGTTT-3' and 3'-ggaattcTTAGTTCCCGAACAACATCACC-5'.

Regulation

Loss of function

Publication

Davidson et al. (2006). FGF signaling delineates the cardiac progenitor field in the simple chordate, Ciona intestinalis. Genes Dev. 20(19):2728-38