Name

Cirobu.Bra>MRFbΔCH (Izzi 2013)

Targeted Gene

(MYF5; MYF6; MYOD1)

Family

Sequence-based

Type

overexpression_construct

Supplier

Comment

Called pTCiMRFbΔCH in the article. In this misexpression plasmid, MRFb (large transcript of MRF) carrying a deletion is cloned upstream of 3.3kb brachyury regulatory element, to target notochord-specific overexpression. The deletion targets the Ci-MRF sequences encoding amino acids 365–385 (HYHH·····CKAC) (this deletion corresponds to bases 1093–1155 beginning with the ATG start codon of CiMRFb).

Regulation

Loss of function

Publication

Izzi et al. (2013). Functional studies of the Ciona intestinalis myogenic regulatory factor reveal conserved features of chordate myogenesis. Dev Biol 376(2):213-23